Proteome-Wide Profiling Using Sample Multiplexing of a Human Cell Line Treated with Cannabidiol (CBD) and Tetrahydrocannabinol (THC).
Delta-9-tetrahydrocannabinol
TMTpro
cannabidiol
endoplasmic reticulum stress
high-field asymmetric ion mobility spectrometry
mitochondrial dysfunction
quantitative proteomics
real-time database searching
tandem mass tags
unfolded protein response
Journal
Proteomes
ISSN: 2227-7382
Titre abrégé: Proteomes
Pays: Switzerland
ID NLM: 101621966
Informations de publication
Date de publication:
02 Nov 2023
02 Nov 2023
Historique:
received:
29
08
2023
revised:
25
10
2023
accepted:
31
10
2023
medline:
21
11
2023
pubmed:
21
11
2023
entrez:
21
11
2023
Statut:
epublish
Résumé
Cannabis has been used historically for both medicinal and recreational purposes, with the most notable cannabinoids being cannabidiol (CBD) and tetrahydrocannabinol (THC). Although their therapeutic effects have been well studied and their recreational use is highly debated, the underlying mechanisms of their biological effects remain poorly defined. In this study, we use isobaric tag-based sample multiplexed proteome profiling to investigate protein abundance differences in the human neuroblastoma SH-SY5Y cell line treated with CBD and THC. We identified significantly regulated proteins by each treatment and performed a pathway classification and associated protein-protein interaction analysis. Our findings suggest that these treatments may lead to mitochondrial dysfunction and induce endoplasmic reticulum stress. These data can potentially be interrogated further to investigate the potential role of CBD and THC in various biological and disease contexts, providing a foundation for future studies.
Identifiants
pubmed: 37987316
pii: proteomes11040036
doi: 10.3390/proteomes11040036
pmc: PMC10661330
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM132129
Pays : United States
Références
J Proteome Res. 2019 Feb 1;18(2):623-632
pubmed: 30450911
Sci Adv. 2022 Feb 25;8(8):eabi6110
pubmed: 35050692
Biochem Pharmacol. 2018 Nov;157:159-168
pubmed: 30134192
Cell Death Dis. 2013 Dec 05;4:e949
pubmed: 24309936
J Proteome Res. 2019 Feb 1;18(2):687-693
pubmed: 30451507
Cells. 2022 Mar 14;11(6):
pubmed: 35326437
Cell Death Dis. 2019 Oct 14;10(10):779
pubmed: 31611561
J Recept Signal Transduct Res. 2021 Aug;41(4):363-370
pubmed: 32862766
BMC Syst Biol. 2014;8 Suppl 4:S11
pubmed: 25521941
Nat Neurosci. 2021 Sep;24(9):1313-1323
pubmed: 34294919
Foods. 2023 Sep 04;12(17):
pubmed: 37685254
Proteomics. 2016 Sep;16(18):2448-53
pubmed: 27461997
J Proteome Res. 2018 Jun 1;17(6):2226-2236
pubmed: 29734811
Proc Natl Acad Sci U S A. 2020 May 5;117(18):9991-10002
pubmed: 32312805
Int J Mol Sci. 2018 Oct 04;19(10):
pubmed: 30287763
Int J Mol Sci. 2020 Nov 12;21(22):
pubmed: 33198323
Mol Neurobiol. 2019 Sep;56(9):6017-6034
pubmed: 30707393
Nat Protoc. 2009;4(1):44-57
pubmed: 19131956
J Pers Med. 2021 Feb 25;11(3):
pubmed: 33668817
J Proteome Res. 2022 Apr 1;21(4):1196-1203
pubmed: 35166117
Nat Biotechnol. 2023 Jun;41(6):845-857
pubmed: 36593396
Braz J Psychiatry. 2013 Oct-Dec;35(4):380-6
pubmed: 24402213
Front Psychol. 2020 May 14;11:833
pubmed: 32528345
Cancer Lett. 2019 Apr 10;447:12-23
pubmed: 30660647
Gen Pharmacol. 1996 Oct;27(7):1141-3
pubmed: 8981058
Proteomics. 2018 Dec;18(23):e1800222
pubmed: 30352137
Int J Environ Res Public Health. 2019 Nov 12;16(22):
pubmed: 31718076
J Steroid Biochem Mol Biol. 2021 Jun;210:105876
pubmed: 33722705
Proteomics. 2022 Oct;22(19-20):e2100247
pubmed: 35866514
Prev Med Rep. 2021 Mar 11;22:101351
pubmed: 33816088
Int J Cancer. 2020 May 15;146(10):2865-2876
pubmed: 31597194
J Mol Neurosci. 2009 Feb;37(2):111-22
pubmed: 18584336
Br J Pharmacol. 2010 Jan;159(1):129-41
pubmed: 20002104
Cell Mol Biol Lett. 2019 Jun 06;24:38
pubmed: 31182966
Cureus. 2020 Jul 21;12(7):e9309
pubmed: 32839678
Cell Death Dis. 2021 Feb 26;12(2):215
pubmed: 33637686
Epilepsy Behav. 2020 Nov;112:107452
pubmed: 32956945
Anal Chem. 2014 Jul 15;86(14):7150-8
pubmed: 24927332
JCI Insight. 2020 Dec 3;5(23):
pubmed: 33141759
Stem Cells. 2013 Dec;31(12):2724-36
pubmed: 23939995
Proc Natl Acad Sci U S A. 2011 Nov 22;108(47):18879-86
pubmed: 22011582
Curr Protoc. 2021 Mar;1(3):e90
pubmed: 33780170
J Neurochem. 2011 Sep;118(6):1101-12
pubmed: 21781118
Oncotarget. 2017 Jan 10;8(2):2585-2593
pubmed: 27906681
Swiss Med Wkly. 2022 Aug 03;152:w30213
pubmed: 35964325
EMBO J. 2014 Apr 1;33(7):668-85
pubmed: 24469251
Nucleic Acids Res. 2021 Jan 8;49(D1):D545-D551
pubmed: 33125081
Nat Methods. 2011 Oct 02;8(11):937-40
pubmed: 21963607
Cell Metab. 2017 Jan 10;25(1):8-10
pubmed: 28076767
J Am Soc Mass Spectrom. 2016 Oct;27(10):1620-5
pubmed: 27400695
Oxid Med Cell Longev. 2017;2017:2969271
pubmed: 29430279
Cell Death Dis. 2019 Nov 7;10(11):846
pubmed: 31699976
Proteomes. 2023 Apr 03;11(2):
pubmed: 37092453
Toxicol Lett. 2014 Nov 18;231(1):62-71
pubmed: 25195527
J Mol Cell Cardiol. 2016 Feb;91:228-37
pubmed: 26778458
J Mol Neurosci. 2012 Feb;46(2):285-92
pubmed: 21603976
Sci Rep. 2021 Mar 8;11(1):5434
pubmed: 33686185
Mol Neurobiol. 2021 Apr;58(4):1695-1710
pubmed: 33237429
Brain Res. 2008 Jan 29;1191:1-11
pubmed: 18163980
J Clin Pharmacol. 2021 Aug;61 Suppl 2:S37-S52
pubmed: 34396558
Neuropathol Appl Neurobiol. 2012 Oct;38(6):535-47
pubmed: 22236282
EMBO Mol Med. 2015 Apr 11;7(6):788-801
pubmed: 25864123
Pharmacology. 2022;107(3-4):131-149
pubmed: 35093949
Urology. 2017 Nov;109:223.e9-223.e16
pubmed: 28827195
Biomed Res Int. 2015;2015:323706
pubmed: 25654095
Reprod Toxicol. 2019 Aug;87:21-31
pubmed: 31054322
Cells. 2021 May 19;10(5):
pubmed: 34069407
J Physiol. 2021 Jan;599(1):23-37
pubmed: 33006160
Oncotarget. 2016 Jul 19;7(29):46603-46614
pubmed: 27366949
J Proteome Res. 2020 May 1;19(5):2026-2034
pubmed: 32126768
Cancers (Basel). 2021 Nov 12;13(22):
pubmed: 34830821
Pathol Res Pract. 2021 Jun;222:153431
pubmed: 34029877
Redox Biol. 2017 Apr;11:577-585
pubmed: 28110213
Cancer Res. 1973 Nov;33(11):2643-52
pubmed: 4748425
Genet Test Mol Biomarkers. 2021 May;25(5):334-345
pubmed: 33970702
Int J Oncol. 2019 Aug;55(2):391-404
pubmed: 31268154
J Neurosci. 2009 Feb 18;29(7):2053-63
pubmed: 19228959
Molecules. 2022 Mar 04;27(5):
pubmed: 35268790
Biomolecules. 2021 Sep 27;11(10):
pubmed: 34680044
Curr Neuropharmacol. 2019;17(10):974-989
pubmed: 31161980
Birth Defects Res. 2022 Nov 1;114(18):1186-1193
pubmed: 35416425
Mol Endocrinol. 2007 Apr;21(4):987-1000
pubmed: 17284663
Mol Neurobiol. 2015 Aug;52(1):26-37
pubmed: 25108670
Anticancer Res. 2018 Aug;38(8):4593-4605
pubmed: 30061226
Science. 2020 Sep 4;369(6508):1155
pubmed: 32883844
Oncotarget. 2019 Jan 01;10(1):45-59
pubmed: 30713602
Int J Mol Sci. 2022 Mar 19;23(6):
pubmed: 35328765
Front Cell Neurosci. 2020 Sep 10;14:179
pubmed: 33132839
Sci Rep. 2017 Oct 4;7(1):12685
pubmed: 28978942
FASEB J. 2020 Aug;34(8):10316-10328
pubmed: 32530114
BJU Int. 2017 Apr;119(4):515-521
pubmed: 28058780
Epilepsia. 2014 Jun;55(6):791-802
pubmed: 24854329
J Alzheimers Dis. 2010;20(4):1069-82
pubmed: 20413890
J Cell Biochem. 2020 Jul 21;:
pubmed: 32692886