Initiation of Plant Virus Infections by Agroinfiltration of Infectious Viral cDNAs (icDNAs).
Agrobacterium tumefaciens
Agroinfiltration
Infectious cDNA
Plant–virus infection
Transient gene expression
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2024
2024
Historique:
medline:
22
11
2023
pubmed:
21
11
2023
entrez:
21
11
2023
Statut:
ppublish
Résumé
Agroinfiltration uses Agrobacterium to deliver T-DNA-based gene expression constructs into plants. This chapter focuses on the standard method, specifically from the perspective of plant virus research, and describes a protocol for the initiation of virus infections in plants via infiltration of Agrobacterium strains carrying infectious viral cDNAs (icDNAs). The method outlines the culture and preparation of Agrobacterium for infiltration, the infiltration procedure, optimization of the optical density of the Agrobacterium suspension, and sampling of infected plants post-agroinfiltration. The advantages of the agroinfiltration method compared to traditional mechanical inoculation using sap from infected plants are discussed. The protocol is applicable for different pathosystems, although case-specific optimization of infiltration parameters and sampling is recommended.
Identifiants
pubmed: 37987896
doi: 10.1007/978-1-0716-3485-1_3
doi:
Substances chimiques
DNA, Complementary
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
33-46Informations de copyright
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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