A growing threat: Investigating the high incidence of benzimidazole fungicides resistance in Iranian Botrytis cinerea isolates.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2023
2023
Historique:
received:
26
06
2023
accepted:
02
11
2023
medline:
23
11
2023
pubmed:
21
11
2023
entrez:
21
11
2023
Statut:
epublish
Résumé
Effective management of fungicide application programs requires monitoring the profile of resistant populations of Botrytis cinerea, given its high-risk nature. This research aimed to examine the sensitivity of 200 B. cinerea isolates collected from different plant species and regions across Iran towards thiophanate-methyl and carbendazim fungicides. To distinguish between susceptible and resistant isolates, the discriminatory dose assay was employed, followed by the selection of representative isolates from each group for EC50 analysis. To identify potential modifications in codon 198 of the β-tubulin gene in B. cinerea resistant isolates, the researchers employed the PCR-RFLP diagnostic method. More than two-thirds of the isolates exhibited a varying degree of resistance to MBC fungicides, even in farms where the application of these fungicides had not taken place in recent years. After treatment with the BsaI enzyme, the PCR product of sensitive isolates displayed two bands measuring 98 and 371 bp, while only one band of 469 bp was identified in resistant isolates. The study also evaluated whether resistance to fungicides could affect the pathogenicity and mycelial growth of the isolates. The findings showed no significant difference between the resistant and sensitive groups in terms of these factors, indicating that resistance does not come at a cost to the pathogen's fitness. Considering the high incidence of resistance and the absence of negative consequences on fitness, it is recommended to exercise caution in the employment of benzimidazole fungicides as part of B. cinerea management strategies.
Identifiants
pubmed: 37988349
doi: 10.1371/journal.pone.0294530
pii: PONE-D-23-19910
pmc: PMC10662737
doi:
Substances chimiques
Fungicides, Industrial
0
Benzimidazoles
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0294530Informations de copyright
Copyright: © 2023 Mobasher Amini et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Déclaration de conflit d'intérêts
The authors declare there is no conflict of interests.
Références
Plant Dis. 2018 Oct;102(10):2016-2024
pubmed: 30133354
Pest Manag Sci. 2013 Jun;69(6):667-78
pubmed: 23576292
Appl Environ Microbiol. 2015 Oct;81(20):7048-56
pubmed: 26231644
Appl Environ Microbiol. 2017 Apr 17;83(9):
pubmed: 28235878
Plant Dis. 2020 Apr;104(4):1069-1075
pubmed: 32027568
Pest Manag Sci. 2009 Feb;65(2):197-204
pubmed: 19051204
Pest Manag Sci. 2002 Sep;58(9):876-88
pubmed: 12233177
Plant Dis. 2018 Sep;102(9):1803-1810
pubmed: 30125196
Appl Environ Microbiol. 2003 Dec;69(12):7145-52
pubmed: 14660360
J Chem Biol. 2014 May 28;7(4):133-41
pubmed: 25320647
Front Microbiol. 2016 Nov 15;7:1814
pubmed: 27895632
Nucleic Acids Res. 1992 Nov 25;20(22):6115-6
pubmed: 1461751
Plant Dis. 2018 Dec;102(12):2578-2585
pubmed: 30299208
J Environ Health Sci Eng. 2017 Apr 24;15:10
pubmed: 28451437