Molecular dynamics of JUNO-IZUMO1 complexation suggests biologically relevant mechanisms in fertilization.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
20 Nov 2023
Historique:
received: 20 07 2023
accepted: 06 11 2023
medline: 23 11 2023
pubmed: 22 11 2023
entrez: 22 11 2023
Statut: epublish

Résumé

JUNO-IZUMO1 binding is the first known physical link created between the sperm and egg membranes in fertilization, however, how this initiates sperm-egg fusion remains elusive. As advanced structural insights will help to combat the infertility crisis, or advance fertility control, we employed all-atom Molecular Dynamics (MD) to derive dynamic structural insights that are difficult to obtain experimentally. We found that the hydrated JUNO-IZUMO1 interface is composed of a large set of short-lived non-covalent interactions. The contact interface is destabilized by strategically located point mutations, as well as by Zn

Identifiants

pubmed: 37990051
doi: 10.1038/s41598-023-46835-0
pii: 10.1038/s41598-023-46835-0
pmc: PMC10663542
doi:

Substances chimiques

Receptors, Cell Surface 0
Membrane Proteins 0
Folic Acid 935E97BOY8
Immunoglobulins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

20342

Subventions

Organisme : Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
ID : 175839

Informations de copyright

© 2023. The Author(s).

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Auteurs

Paulina Pacak (P)

Department of Health Sciences and Technology, ETH Zurich, Zurich, Switzerland.

Carleen Kluger (C)

Lehrstuhl für Angewandte Physik and Center for NanoScience, Ludwig-Maximilians-Universität, München, Munich, Germany.
Evotec München GmbH, Neuried, Germany.

Viola Vogel (V)

Department of Health Sciences and Technology, ETH Zurich, Zurich, Switzerland. viola.vogel@hest.ethz.ch.

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