Synergistic Pharmacological Therapy to Modulate Glial Cells in Spinal Cord Injury.
glial cells
nanoparticles
spinal cord injury
super-resolution microscopy
Journal
Advanced materials (Deerfield Beach, Fla.)
ISSN: 1521-4095
Titre abrégé: Adv Mater
Pays: Germany
ID NLM: 9885358
Informations de publication
Date de publication:
22 Nov 2023
22 Nov 2023
Historique:
revised:
10
11
2023
received:
02
08
2023
pubmed:
22
11
2023
medline:
22
11
2023
entrez:
22
11
2023
Statut:
aheadofprint
Résumé
Current treatments for modulating the glial-mediated inflammatory response after spinal cord injury (SCI) have limited ability to improve recovery. This is quite likely due to the lack of a selective therapeutic approach acting on microgliosis and astrocytosis, the glia components most involved after trauma, while maximizing efficacy and minimizing side effects. A new nanogel that can selectively release active compounds in microglial cells and astrocytes is developed and characterized. The degree of selectivity and subcellular distribution of the nanogel is evaluated by applying an innovative super-resolution microscopy technique, expansion microscopy. Two different administration schemes are then tested in a SCI mouse model: in an early phase, the nanogel loaded with Rolipram, an anti-inflammatory drug, achieves significant improvement in the animal's motor performance due to the increased recruitment of microglia and macrophages that are able to localize the lesion. Treatment in the late phase, however, gives opposite results, with worse motor recovery because of the widespread degeneration. These findings demonstrate that the nanovector can be selective and functional in the treatment of the glial component in different phases of SCI. They also open a new therapeutic scenario for tackling glia-mediated inflammation after neurodegenerative events in the central nervous system.
Identifiants
pubmed: 37990971
doi: 10.1002/adma.202307747
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2307747Subventions
Organisme : Politecnico di Milano
ID : CNRBIOMICS PON R&I
Organisme : Politecnico di Milano
ID : PIR01_00017
Informations de copyright
© 2023 The Authors. Advanced Materials published by Wiley-VCH GmbH.
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