Optogenetic-mediated induction and monitoring of α-synuclein aggregation in cellular models of Parkinson's disease.

Cell Biology Microscopy Neuroscience

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
21 Nov 2023
Historique:
received: 10 08 2023
revised: 09 10 2023
accepted: 06 11 2023
medline: 22 11 2023
pubmed: 22 11 2023
entrez: 22 11 2023
Statut: aheadofprint

Résumé

Studying Parkinson's disease (PD) is complex due to a lack of cellular models mimicking key aspects of protein pathology. Here, we present a protocol for inducing and monitoring α-synuclein aggregation in living cells using optogenetics. We describe steps for plasmid transduction, biochemical validation, immunocytochemistry, and live-cell confocal imaging. These induced aggregates fulfill the cardinal features of authentic protein inclusions observed in PD-diseased brains and offer a tool to study the role of protein aggregation in neurodegeneration. For complete details on the use and execution of this protocol, please refer to Bérard et al.

Identifiants

pubmed: 37991922
pii: S2666-1667(23)00705-0
doi: 10.1016/j.xpro.2023.102738
pmc: PMC10700619
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

102738

Informations de copyright

Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

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Auteurs

Maxime Teixeira (M)

CHU de Québec Research Center, Axe Neurosciences, Quebec City, Canada; Department of Molecular Medicine, Faculty of Medicine, Université Laval, Quebec City, Canada. Electronic address: maxime.teixeira@crchudequebec.ulaval.ca.

Razan Sheta (R)

CHU de Québec Research Center, Axe Neurosciences, Quebec City, Canada; Department of Molecular Medicine, Faculty of Medicine, Université Laval, Quebec City, Canada.

Walid Idi (W)

CHU de Québec Research Center, Axe Neurosciences, Quebec City, Canada; Department of Molecular Medicine, Faculty of Medicine, Université Laval, Quebec City, Canada.

Abid Oueslati (A)

CHU de Québec Research Center, Axe Neurosciences, Quebec City, Canada; Department of Molecular Medicine, Faculty of Medicine, Université Laval, Quebec City, Canada. Electronic address: abid.oueslati.1@ulaval.ca.

Classifications MeSH