Optogenetic-mediated induction and monitoring of α-synuclein aggregation in cellular models of Parkinson's disease.
Cell Biology
Microscopy
Neuroscience
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
21 Nov 2023
21 Nov 2023
Historique:
received:
10
08
2023
revised:
09
10
2023
accepted:
06
11
2023
medline:
22
11
2023
pubmed:
22
11
2023
entrez:
22
11
2023
Statut:
aheadofprint
Résumé
Studying Parkinson's disease (PD) is complex due to a lack of cellular models mimicking key aspects of protein pathology. Here, we present a protocol for inducing and monitoring α-synuclein aggregation in living cells using optogenetics. We describe steps for plasmid transduction, biochemical validation, immunocytochemistry, and live-cell confocal imaging. These induced aggregates fulfill the cardinal features of authentic protein inclusions observed in PD-diseased brains and offer a tool to study the role of protein aggregation in neurodegeneration. For complete details on the use and execution of this protocol, please refer to Bérard et al.
Identifiants
pubmed: 37991922
pii: S2666-1667(23)00705-0
doi: 10.1016/j.xpro.2023.102738
pmc: PMC10700619
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
102738Informations de copyright
Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
Références
Curr Protoc Cell Biol. 2018 Jun;79(1):e51
pubmed: 29924488
J Vis Exp. 2008 Jul 16;(17):
pubmed: 19066511
Nat Protoc. 2006;1(1):241-5
pubmed: 17406239
Methods Enzymol. 2006;412:33-48
pubmed: 17046650
Neuron. 2014 Jun 18;82(6):1271-88
pubmed: 24857020
STAR Protoc. 2023 Jul 28;4(3):102486
pubmed: 37515763
Nat Commun. 2014 Sep 18;5:4925
pubmed: 25233328
PLoS Biol. 2022 Mar 9;20(3):e3001578
pubmed: 35263320
Biochemistry. 2020 Dec 8;59(48):4563-4572
pubmed: 33237763
Hum Gene Ther Methods. 2012 Aug;23(4):242-54
pubmed: 22934828
Methods Enzymol. 2010;470:709-34
pubmed: 20946833
PLoS One. 2008 Apr 02;3(4):e1867
pubmed: 18382657
Hum Gene Ther. 2000 Jan 1;11(1):179-90
pubmed: 10646649