Development of a real-time enzymatic recombinase amplification assay for rapid detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in shrimp Penaeus vannamei.

Endogenous viral elements Enzymatic recombinase amplification Infectious hypodermal and hematopoietic necrosis virus Penaeus vannamei

Journal

Journal of invertebrate pathology
ISSN: 1096-0805
Titre abrégé: J Invertebr Pathol
Pays: United States
ID NLM: 0014067

Informations de publication

Date de publication:
Nov 2023
Historique:
received: 01 10 2023
revised: 18 11 2023
accepted: 18 11 2023
pubmed: 23 11 2023
medline: 23 11 2023
entrez: 22 11 2023
Statut: ppublish

Résumé

Infectious hypodermal and hematopoietic necrosis virus (IHHNV) is classified as a reportable crustacean disease by the World Organisation for Animal Health (WOAH), which causes poor growth in Penaeus vannamei. According to genome sequence alignment analysis, enzymatic recombinase amplification (ERA) primers and probe were designed based on the ORF1 region of IHHNV, and a real-time ERA assay for IHHNV detection (IHHNV-ERA) was established. The experimental results show that IHHNV-F2/IHHNV-R2 and IHHNV-Probe can effectively amplify the target gene, and the sensitivity is 1.4 × 10

Identifiants

DOI: 10.1016/j.jip.2023.108024 PMID: 37992986
pubmed: 37992986
pii: S0022-2011(23)00141-6
doi: 10.1016/j.jip.2023.108024
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

108024

Informations de copyright

Copyright © 2023 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Lu Zhang (L)

MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao 266003, China; Key Laboratory of Tropical Aquatic Germplasm of Hainan Province, Sanya Oceanographic Institution, Ocean University of China, Sanya 572024, China.

Kexin Liu (K)

MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao 266003, China; Key Laboratory of Tropical Aquatic Germplasm of Hainan Province, Sanya Oceanographic Institution, Ocean University of China, Sanya 572024, China.

Mengran Liu (M)

MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao 266003, China; Key Laboratory of Tropical Aquatic Germplasm of Hainan Province, Sanya Oceanographic Institution, Ocean University of China, Sanya 572024, China; Hainan Yazhou Bay Seed Laboratory, Sanya 572024, China. Electronic address: lmr@ouc.edu.cn.

Jingjie Hu (J)

MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao 266003, China; Key Laboratory of Tropical Aquatic Germplasm of Hainan Province, Sanya Oceanographic Institution, Ocean University of China, Sanya 572024, China; Hainan Yazhou Bay Seed Laboratory, Sanya 572024, China.

Zhenmin Bao (Z)

MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao 266003, China; Key Laboratory of Tropical Aquatic Germplasm of Hainan Province, Sanya Oceanographic Institution, Ocean University of China, Sanya 572024, China; Hainan Yazhou Bay Seed Laboratory, Sanya 572024, China.

Mengqiang Wang (M)

MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao 266003, China; Key Laboratory of Tropical Aquatic Germplasm of Hainan Province, Sanya Oceanographic Institution, Ocean University of China, Sanya 572024, China; Hainan Yazhou Bay Seed Laboratory, Sanya 572024, China. Electronic address: wangmengqiang@ouc.edu.cn.

Classifications MeSH