Transcriptomic profiles reveal the characteristics of oocytes and cumulus cells at GV, MI, and MII in follicles before ovulation.
Cumulus cell
Oocyte
Oocyte maturation
Transcriptome
Journal
Journal of ovarian research
ISSN: 1757-2215
Titre abrégé: J Ovarian Res
Pays: England
ID NLM: 101474849
Informations de publication
Date de publication:
22 Nov 2023
22 Nov 2023
Historique:
received:
11
05
2023
accepted:
01
10
2023
medline:
24
11
2023
pubmed:
23
11
2023
entrez:
23
11
2023
Statut:
epublish
Résumé
The oocyte and its surrounding cumulus cells (CCs) exist as an inseparable entity. The maturation of the oocyte relies on communication between the oocyte and the surrounding CCs. However, oocyte evaluation is primarily based on morphological parameters currently, which offer limited insight into the quality and competence of the oocyte. Here, we conducted transcriptomic profiling of oocytes and their CCs from 47 patients undergoing preimplantation genetic testing for aneuploidy (PGT-A). We aimed to investigate the molecular events occurring between oocytes and CCs at different stages of oocyte maturation (germinal vesicle [GV], metaphase I [MI], and metaphase II [MII]). Our goal is to provide new insights into in vitro oocyte maturation (IVM). Our findings indicate that oocyte maturation is a complex and dynamic process and that MI oocytes can be further classified into two distinct subtypes: GV-like-MI oocytes and MII-like-MI oocytes. Human oocytes and cumulus cells at three different stages of maturation were analyzed using RNA-seq, which revealed unique transcriptional machinery, stage-specific genes and pathways, and transcription factor networks that displayed developmental stage-specific expression patterns. We have also identified that both lipid and cholesterol metabolism in cumulus cells is active during the late stage of oocyte maturation. Lipids may serve as a more efficient energy source for oocytes and even embryogenesis. Overall, our study provides a relatively comprehensive overview of the transcriptional characteristics and potential interactions between human oocytes and cumulus cells at various stages of maturation before ovulation. This study may offer novel perspectives on IVM and provide a reliable reference data set for understanding the transcriptional regulation of follicular maturation.
Sections du résumé
BACKGROUND
BACKGROUND
The oocyte and its surrounding cumulus cells (CCs) exist as an inseparable entity. The maturation of the oocyte relies on communication between the oocyte and the surrounding CCs. However, oocyte evaluation is primarily based on morphological parameters currently, which offer limited insight into the quality and competence of the oocyte. Here, we conducted transcriptomic profiling of oocytes and their CCs from 47 patients undergoing preimplantation genetic testing for aneuploidy (PGT-A). We aimed to investigate the molecular events occurring between oocytes and CCs at different stages of oocyte maturation (germinal vesicle [GV], metaphase I [MI], and metaphase II [MII]). Our goal is to provide new insights into in vitro oocyte maturation (IVM).
RESULTS
RESULTS
Our findings indicate that oocyte maturation is a complex and dynamic process and that MI oocytes can be further classified into two distinct subtypes: GV-like-MI oocytes and MII-like-MI oocytes. Human oocytes and cumulus cells at three different stages of maturation were analyzed using RNA-seq, which revealed unique transcriptional machinery, stage-specific genes and pathways, and transcription factor networks that displayed developmental stage-specific expression patterns. We have also identified that both lipid and cholesterol metabolism in cumulus cells is active during the late stage of oocyte maturation. Lipids may serve as a more efficient energy source for oocytes and even embryogenesis.
CONCLUSIONS
CONCLUSIONS
Overall, our study provides a relatively comprehensive overview of the transcriptional characteristics and potential interactions between human oocytes and cumulus cells at various stages of maturation before ovulation. This study may offer novel perspectives on IVM and provide a reliable reference data set for understanding the transcriptional regulation of follicular maturation.
Identifiants
pubmed: 37993893
doi: 10.1186/s13048-023-01291-2
pii: 10.1186/s13048-023-01291-2
pmc: PMC10664256
doi:
Types de publication
Review
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
225Subventions
Organisme : the National Key R&D Program of China
ID : grant 2018YFC1003100, to L.H.
Organisme : the National Natural Science Foundation of China
ID : grant 81873478, to L.H.
Informations de copyright
© 2023. The Author(s).
Références
Int J Mol Sci. 2018 Oct 20;19(10):
pubmed: 30347829
Mol Cell Endocrinol. 2005 Apr 29;234(1-2):37-45
pubmed: 15836951
Mol Cell Endocrinol. 2006 Apr 25;249(1-2):64-70
pubmed: 16500744
Biol Reprod. 2004 Mar;70(3):535-47
pubmed: 14613897
Biol Reprod. 1998 Aug;59(2):437-43
pubmed: 9687319
Mol Hum Reprod. 2010 Oct;16(10):715-25
pubmed: 20435609
J Assist Reprod Genet. 2004 Mar;21(3):79-83
pubmed: 15202735
Biol Reprod. 2021 Apr 1;104(4):902-913
pubmed: 33480981
Biochim Biophys Acta. 2012 Dec;1822(12):1896-912
pubmed: 22634430
Mol Cell Endocrinol. 2020 Oct 1;516:110952
pubmed: 32712385
Dev Biol. 2001 May 15;233(2):258-70
pubmed: 11336494
Dev Biol. 1994 Mar;162(1):154-68
pubmed: 8125183
Fertil Steril. 2017 Apr;107(4):840-847
pubmed: 28292619
J Cell Physiol. 2018 Sep;233(9):6952-6964
pubmed: 29336483
J Ovarian Res. 2020 Aug 12;13(1):93
pubmed: 32787963
Mol Cell. 2018 Dec 20;72(6):1021-1034.e4
pubmed: 30472193
J Pineal Res. 2017 Mar;62(2):
pubmed: 28095627
Theriogenology. 2012 May;77(8):1632-41
pubmed: 22365693
Biol Reprod. 1994 Feb;50(2):266-70
pubmed: 8142545
Biol Reprod. 2011 Jul;85(1):62-9
pubmed: 21311036
Mol Hum Reprod. 2012 Dec;18(12):572-84
pubmed: 22923488
Hum Reprod. 2007 May;22(5):1239-46
pubmed: 17303631
J Endocrinol. 2006 Jan;188(1):111-9
pubmed: 16394180
BMC Bioinformatics. 2006 Mar 20;7 Suppl 1:S7
pubmed: 16723010
J Exp Zool. 1985 May;234(2):231-6
pubmed: 3998681
J Cell Physiol. 2018 Jan;233(1):302-312
pubmed: 28240360
Biol Reprod. 2013 May 02;88(5):111
pubmed: 23536372
Curr Opin Cell Biol. 2015 Apr;33:119-24
pubmed: 25703629
Mol Hum Reprod. 2009 Jan;15(1):11-7
pubmed: 19038973
Biol Reprod. 2013 Jun 27;88(6):164
pubmed: 23616596
Biol Reprod. 2021 Jan 4;104(1):106-116
pubmed: 33404651
Biol Reprod. 2011 Jun;84(6):1248-57
pubmed: 21293029
Hum Reprod Update. 2008 Mar-Apr;14(2):159-77
pubmed: 18175787
Reproduction. 2010 Apr;139(4):685-95
pubmed: 20089664
Dev Biol. 1994 Jul;164(1):1-9
pubmed: 8026614
Reproduction. 2001 May;121(5):647-53
pubmed: 11427152
Anim Reprod Sci. 2004 Jul;82-83:431-46
pubmed: 15271471
Hum Reprod. 1997 Nov;12(11 Suppl):127-32
pubmed: 9433969
Reprod Biomed Online. 2014 Mar;28(3):284-99
pubmed: 24444815
Int J Mol Sci. 2021 Mar 26;22(7):
pubmed: 33810351
J Lipid Res. 2010 Aug;51(8):2362-71
pubmed: 20404351
Cell Death Dis. 2019 Jul 22;10(8):558
pubmed: 31332164
Reproduction. 2017 Feb;153(2):R69-R83
pubmed: 27815559
Nucleic Acids Res. 2012 Jan;40(Database issue):D144-9
pubmed: 22080564
J Zhejiang Univ Sci B. 2009 Jul;10(7):483-92
pubmed: 19585665
Hum Reprod. 2008 Mar;23(3):504-13
pubmed: 18216034
Proc Natl Acad Sci U S A. 1967 Aug;58(2):560-7
pubmed: 5233459