Inserting a Neuropixels probe into awake monkey cortex: two probes, two methods.

Chronic recording chamber High-density silicon probe Multi-contact linear probes Nonhuman primate neurophysiology

Journal

Journal of neuroscience methods
ISSN: 1872-678X
Titre abrégé: J Neurosci Methods
Pays: Netherlands
ID NLM: 7905558

Informations de publication

Date de publication:
22 Nov 2023
Historique:
received: 27 06 2023
revised: 17 10 2023
accepted: 18 11 2023
pubmed: 24 11 2023
medline: 24 11 2023
entrez: 23 11 2023
Statut: aheadofprint

Résumé

Neuropixels probes have revolutionized neurophysiological studies in the rodent, but inserting these probes through the much thicker primate dura remains a challenge. Here we describe two methods we have developed for the insertion of two types of Neuropixels probes acutely into the awake macaque monkey cortex. For the fine rodent probe (Neuropixels 1.0, IMEC), which is unable to pierce native primate dura, we developed a dural-eyelet method to insert the probe repeatedly without breakage. For the thicker short NHP probe (Neuropixels NP1010), we developed an artificial dura system to insert the probe. We have now conducted successful experiments in 3 animals across 7 recording chambers with the procedures described here and have achieved recordings with similar yields over several months in each case. We hope that our hardware, surgical preparation, methods for insertion and methods for removal of broken probe parts are of value to primate physiologists everywhere.

Sections du résumé

BACKGROUND BACKGROUND
Neuropixels probes have revolutionized neurophysiological studies in the rodent, but inserting these probes through the much thicker primate dura remains a challenge.
NEW METHODS METHODS
Here we describe two methods we have developed for the insertion of two types of Neuropixels probes acutely into the awake macaque monkey cortex. For the fine rodent probe (Neuropixels 1.0, IMEC), which is unable to pierce native primate dura, we developed a dural-eyelet method to insert the probe repeatedly without breakage. For the thicker short NHP probe (Neuropixels NP1010), we developed an artificial dura system to insert the probe.
RESULTS AND COMPARISON WITH EXISTING METHODS RESULTS
We have now conducted successful experiments in 3 animals across 7 recording chambers with the procedures described here and have achieved recordings with similar yields over several months in each case.
CONCLUSION CONCLUSIONS
We hope that our hardware, surgical preparation, methods for insertion and methods for removal of broken probe parts are of value to primate physiologists everywhere.

Identifiants

DOI: 10.1016/j.jneumeth.2023.110016 PMID: 37995854
pubmed: 37995854
pii: S0165-0270(23)00235-2
doi: 10.1016/j.jneumeth.2023.110016
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

110016

Commentaires et corrections

Type : UpdateOf

Informations de copyright

Copyright © 2023 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare that they have no competing interests.

Auteurs

Tomoyuki Namima (T)

Department of Biological Structure and Washington National Primate Research Center, University of Washington, Seattle, WA 98195, USA; Graduate School of Frontier Biosciences, Osaka University, and Center for Information and Neural Networks, National Institute of Information and Communications Technology, Suita, Osaka 565-0871, Japan.

Erin Kempkes (E)

Department of Biological Structure and Washington National Primate Research Center, University of Washington, Seattle, WA 98195, USA.

Bob Smith (B)

Washington National Primate Research Center Instrumentation Services, University of Washington, Seattle, WA 98195, USA.

Lydia Smith (L)

Washington National Primate Research Center, University of Washington, Seattle, WA 98195, USA.

Amy L Orsborn (AL)

Washington National Primate Research Center, University of Washington, Seattle, WA 98195, USA.

Anitha Pasupathy (A)

Department of Biological Structure and Washington National Primate Research Center, University of Washington, Seattle, WA 98195, USA; Washington National Primate Research Center, University of Washington, Seattle, WA 98195, USA. Electronic address: pasupat@u.washington.edu.

Classifications MeSH