Vector-borne Trypanosoma brucei parasites develop in artificial human skin and persist as skin tissue forms.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
23 Nov 2023
23 Nov 2023
Historique:
received:
10
07
2021
accepted:
08
11
2023
medline:
27
11
2023
pubmed:
24
11
2023
entrez:
23
11
2023
Statut:
epublish
Résumé
Transmission of Trypanosoma brucei by tsetse flies involves the deposition of the cell cycle-arrested metacyclic life cycle stage into mammalian skin at the site of the fly's bite. We introduce an advanced human skin equivalent and use tsetse flies to naturally infect the skin with trypanosomes. We detail the chronological order of the parasites' development in the skin by single-cell RNA sequencing and find a rapid activation of metacyclic trypanosomes and differentiation to proliferative parasites. Here we show that after the establishment of a proliferative population, the parasites enter a reversible quiescent state characterized by slow replication and a strongly reduced metabolism. We term these quiescent trypanosomes skin tissue forms, a parasite population that may play an important role in maintaining the infection over long time periods and in asymptomatic infected individuals.
Identifiants
pubmed: 37996412
doi: 10.1038/s41467-023-43437-2
pii: 10.1038/s41467-023-43437-2
pmc: PMC10667367
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
7660Subventions
Organisme : Deutsche Forschungsgemeinschaft (German Research Foundation)
ID : EN305
Organisme : Deutsche Forschungsgemeinschaft (German Research Foundation)
ID : SPP1726
Organisme : Deutsche Forschungsgemeinschaft (German Research Foundation)
ID : SPP2332
Organisme : German-Israeli Foundation for Scientific Research and Development (GIF)
ID : I-473-416.13/2018
Organisme : EC | Horizon 2020 Framework Programme (EU Framework Programme for Research and Innovation H2020)
ID : 955910
Informations de copyright
© 2023. The Author(s).
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