Porcine UL-16 Binding Protein 1 Is Not a Functional Ligand for the Human Natural Killer Cell Activating Receptor NKG2D.


Journal

Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052

Informations de publication

Date de publication:
07 Nov 2023
Historique:
received: 01 10 2023
revised: 06 11 2023
accepted: 06 11 2023
medline: 27 11 2023
pubmed: 24 11 2023
entrez: 24 11 2023
Statut: epublish

Résumé

Natural killer (NK) cells play a vital role in xenotransplantation rejection. One approach to induce NK cell immune tolerance is to prevent the NK cell-mediated direct killing of porcine cells by targeting the interaction of the activating receptor NKG2D and its ligands. However, the identity of porcine ligands for the human NKG2D receptor has remained elusive. Previous studies on porcine UL-16 binding protein 1 (pULBP-1) as a ligand for human NKG2D have yielded contradictory results. The goal of the present study was to clarify the role of pULBP-1 in the immune response and its interaction with human NKG2D receptor. To accomplish this, the CRISPR/Cas9 gene editing tool was employed to disrupt the porcine

Identifiants

pubmed: 37998322
pii: cells12222587
doi: 10.3390/cells12222587
pmc: PMC10670462
pii:
doi:

Substances chimiques

Receptors, Natural Killer Cell 0
NK Cell Lectin-Like Receptor Subfamily K 0
Ligands 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NCI NIH HHS
ID : P30 CA082709
Pays : United States
Organisme : NIDDK NIH HHS
ID : U54 DK106846
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI164002
Pays : United States

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Auteurs

Kevin J Lopez (KJ)

Department of Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USA.

John Paul Spence (JP)

Department of Pediatrics, Indiana University School of Medicine, Indianapolis, IN 46202, USA.

Wei Li (W)

Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN 46202, USA.

Wenjun Zhang (W)

Department of Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USA.

Barry Wei (B)

Department of Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USA.

Arthur A Cross-Najafi (AA)

Department of Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USA.

James R Butler (JR)

Department of Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USA.

David K C Cooper (DKC)

Center for Transplantation Sciences, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA.

Burcin Ekser (B)

Department of Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USA.

Ping Li (P)

Department of Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USA.

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Classifications MeSH