In vitro and ex vivo anti-Pythium insidiosum potential of ozonated sunflower oil.
Oomycete
Ozone
Ozone therapy
P. insidiosum
Sunflower oil
Vegetable oil
Journal
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
ISSN: 1678-4405
Titre abrégé: Braz J Microbiol
Pays: Brazil
ID NLM: 101095924
Informations de publication
Date de publication:
24 Nov 2023
24 Nov 2023
Historique:
received:
07
03
2023
accepted:
27
10
2023
medline:
24
11
2023
pubmed:
24
11
2023
entrez:
24
11
2023
Statut:
aheadofprint
Résumé
This study sought to evaluate the in vitro and ex vivo susceptibility of Pythium insidiosum to ozonized sunflower oil (OSO) and verify the morphological alterations of OSO-exposed hyphae. Susceptibility assays were performed according to the broth microdilution protocol M38-A2/CLSI, and the minimal inhibitory (MIC) and minimal oomicidal (MOC) concentrations were also determined. Non-ozonated sunflower oil (SO) was used as the oil control. Additionally, kunkers from equine pythiosis were exposed to OSO. Damages caused by OSO and SO on P. insidiosum hyphae ultrastructure were verified using scanning electron microscopy. The MIC range for OSO was 7000 to 437.5 mg/mL, and the values for SO were higher, ranging from 56000 to 14000 mg/mL. The MOC was equal to MIC for both oil formulations. The OSO fully inhibited the oomycete growth from kunkers, although there was P. insidiosum growth in the kunker control in 24 h of incubation. The SEM analyses showed that both OSO and SO caused morphological alterations in P. insidiosum hyphae, highlighting the presence of cavitation along the hyphae with loss of continuity of the cell wall, which was more evident in the OSO-treated hyphae. The OSO had the best oomicidal activity, leading us to believe that our findings may support future research containing this formulation to be applied in integrative medicine protocols to control pythiosis in animals and humans.
Identifiants
pubmed: 37999913
doi: 10.1007/s42770-023-01173-1
pii: 10.1007/s42770-023-01173-1
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2023. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.
Références
Gaastra W, Lipman LJ, De Cock AW et al (2010) Pythium insidiosum: an overview. Vet Microbiol 146:1–16. https://doi.org/10.1016/j.vetmic.2010.07.019
doi: 10.1016/j.vetmic.2010.07.019
pubmed: 20800978
Yolanda H, Krajaejun T (2020) Review of methods and antimicrobial agents for susceptibility testing against Pythium insidiosum. Heliyon 6:e03737. https://doi.org/10.1016/j.heliyon.2020.e03737
doi: 10.1016/j.heliyon.2020.e03737
pubmed: 32322727
pmcid: 7160450
Mendoza L, Vilela R (2013) The mamalian pathogenic oomycetes. Curr Fungal Infect Rep 7:198–208. https://doi.org/10.1016/j.funbio.2014.05.004
doi: 10.1016/j.funbio.2014.05.004
Lerksuthirat T, Sangcakul A, Lohnoo T et al (2017) Evolution of the sterol biosynthetic pathway of Pythium insidiosum and related oomycetes contributes to antifungal drug resistance. Antimicrob Agents Chemother 61:e02352-e2416. https://doi.org/10.1128/AAC.02352-16
doi: 10.1128/AAC.02352-16
pubmed: 28115356
pmcid: 5365696
Loreto ES, Tondolo JS, Pilotto MB et al (2014) New insights into the in vitro susceptibility of Pythium insidiosum. Antimicrob Agents Chemother 58:7534–7537. https://doi.org/10.1128/AAC.02680-13
doi: 10.1128/AAC.02680-13
pubmed: 25223997
pmcid: 4249561
Loreto ES, Tondolo JSM, Oliveira DC et al (2018) In vitro activities of miltefosine and antibacterial agents from the macrolide, oxazolidinone, and pleuromutilin classes against Pythium insidiosum and Pythium aphanidermatum. Antimicrob Agents Chemother 62:e01678-e1717. https://doi.org/10.1128/AAC.01678-17
doi: 10.1128/AAC.01678-17
pubmed: 29311087
pmcid: 5826131
Krajaejun T, Lohnoo T, Yingyong W et al (2019) The repurposed drug disulfiram inhibits urease and aldehyde dehydrogenase and prevents in vitro growth of the oomycete Pythium insidiosum. Antimicrob Agents Chemother 63:e00609-19. https://doi.org/10.1128/AAC.00609-19
doi: 10.1128/AAC.00609-19
pubmed: 31138572
pmcid: 6658754
Fonseca AOS, Pereira DIB, Jacob RG et al (2015) In vitro susceptibility of Brazilian Pythium insidiosum isolates to essential oils of some Lamiaceae family species. Mycopathologia 179:253–258. https://doi.org/10.1007/s11046-014-9841-6
doi: 10.1007/s11046-014-9841-6
pubmed: 25431090
Valente JSS, Fonseca AOS, Brasil CL et al (2016) In vitro activity of Melaleuca alternifolia (Tea Tree) in its free oil and nanoemulsion formulations against Pythium insidiosum. Mycopathologia 181:865–869. https://doi.org/10.1007/s11046-016-0051-2
doi: 10.1007/s11046-016-0051-2
Sriphana U, Thongsri Y, Ardwichai P et al (2013) New lignin esters from Alyxia schlechteri and antifungal activity against Pythium insidiosum. Fitoterapia 91:39–43. https://doi.org/10.1016/j.fitote.2013.08.005
doi: 10.1016/j.fitote.2013.08.005
pubmed: 23994626
Sriphana U, Thongsri Y, Prariyachatigul C et al (2013) Clauraila E from the roots of Clausena harmandiana and antifungal activity against Pythium insidiosum. Arch Pharm Res 36:1078–1083. https://doi.org/10.1007/s12272-013-0115-5
doi: 10.1007/s12272-013-0115-5
pubmed: 23595552
Suthiwong J, Sriphana U, Thongsri Y et al (2014) Coumarinoids from the fruits of Micromelum facatum. Fitoterapia 94:134–141. https://doi.org/10.1016/j.fitote.2014.02.004
doi: 10.1016/j.fitote.2014.02.004
pubmed: 24561007
Trolezi R, Azanha JM, Paschoal NR et al (2017) Stryphnodendron adstringens and purified tannin on Pythium insidiosum: in vitro and in vivo studies. Ann Clin Microbiol Antimicrob 16:1–7. https://doi.org/10.1016/j.fitote.2014.02.004
doi: 10.1016/j.fitote.2014.02.004
Wittayapipath K, Yenjai C, Prariyachatigul C et al (2020) Evaluation of antifungal effect and toxicity of xanthyletin and two bacterial metabolites against Thai isolates of Pythium insidiosum. Sci Rep 10:4495. https://doi.org/10.1038/s41598-020-61271-0
doi: 10.1038/s41598-020-61271-0
pubmed: 32161276
pmcid: 7066183
Silveira JS, Brasil CL, Braga CQ et al (2022) Melaleuca alternifolia formulations in the treatment of experimental pythiosis. Braz J Microbiol 53:1011–1017. https://doi.org/10.1007/s42770-022-00720-6
doi: 10.1007/s42770-022-00720-6
Valente JSS, Brasil CL, Braga CQ et al (2020) Biogenic silver nanoparticles in the treatment of experimental pythiosis Bio-AgNP in pythiosis therapy. Med Mycol 58:913–918. https://doi.org/10.1093/mmy/myz141
doi: 10.1093/mmy/myz141
pubmed: 32030424
Ianiski LB, Maciel AF, Weiblen C et al (2022) Oomicidal activity of polypyrrole nanoparticles against Pythium insidiosum. Lett Appl Microbiol 76:1–4. https://doi.org/10.1093/lambio/ovac020
doi: 10.1093/lambio/ovac020
Liu L, Zeng L, Gao L et al (2022) Ozone therapy for skin diseases: Cellular and molecular mechanisms. Int Wound J 10.111:iwj14060. https://doi.org/10.1111/iwj.14060
doi: 10.1111/iwj.14060
Sciorsci RL, Lillo E, Occhiogrosso L et al (2020) Ozone therapy in veterinary medicine: A review. Res Vet Sci 130:240–246. https://doi.org/10.1016/j.rvsc.2020.03.026
doi: 10.1016/j.rvsc.2020.03.026
pubmed: 32234614
Moureu S, Violleau F, Haimoud-Lekhal DA, Calmon (2016) A Influence of storage temperature on the composition and the antibacterial activity of ozonized sunflower oil. Ozone: Science & Engineering 38:143–149. https://doi.org/10.1080/01919512.2015.1128319
doi: 10.1080/01919512.2015.1128319
Kogawa NRA, De Arruda EJ, Micheletti AC et al (2015) Synthesis, characterization, thermal behavior, and biological activity of ozonides from vegetable oils. RSC Adv 5:65427–65436. https://doi.org/10.1039/C5RA02798E
doi: 10.1039/C5RA02798E
Ugazio E, Tullio V, Binello A et al (2020) Ozonated oils as antimicrobial systems in topical applications. Their characterization, current applications, and advances in improved delivery techniques. Molecules 25:334. https://doi.org/10.3390/molecules25020334
doi: 10.3390/molecules25020334
pubmed: 31947580
pmcid: 7024311
Song M, Zeng Q, Xiang Y et al (2018) The antibacterial effect of topical ozone on the treatment of MRSA skin infection. Mol Med Rep 17:2449–2455. https://doi.org/10.3892/mmr.2017.8148
doi: 10.3892/mmr.2017.8148
pubmed: 29207120
Ouf SA, Moussa TA, Abd-Elmegeed AM et al (2016) Anti-fungal potential of ozone against some dermatophytes. Braz J Microbiol 47:697–702. https://doi.org/10.1016/j.bjm.2016.04.014
doi: 10.1016/j.bjm.2016.04.014
pubmed: 27287337
pmcid: 4927674
Tizaoui C (2020) Ozone: A potential oxidant for COVID-19 Virus (SARS-CoV-2). Ozone Sci Eng 42:378–385. https://doi.org/10.1080/01919512.2020.1795614
doi: 10.1080/01919512.2020.1795614
Ferreira JC, Pires RH, Costa GB et al (2021) The in vitro effect of ozone therapy against equine Pythium insidiosum. J Equine Vet Sci 98:103305. https://doi.org/10.1016/j.jevs.2020.103305
doi: 10.1016/j.jevs.2020.103305
pubmed: 33663716
Azevedo MI, Pereira DIB, Botton SA et al (2012) Pythium insidiosum: morphological and molecular identification of Brazilian isolates. Pesq Vet Bras 32:619–622. https://doi.org/10.1590/S0100-736X2012000700005
doi: 10.1590/S0100-736X2012000700005
Weiblen C, Azevedo MI, Ianiski LB et al (2019) Genotyping of South American clinical isolates of Pythium insidiosum based on single nucleotide polymorphism-based multiplex PCR. Cienc Rural 49:e20180744. https://doi.org/10.1590/0103-8478cr20180744
doi: 10.1590/0103-8478cr20180744
Fonseca AOS, Pereira DIB, Filho FSM et al (2014) In vitro susceptibility of zoospores and hyphae of Pythium insidiosum to antifungals. J Antimicrob Chemother 69:1564–1567. https://doi.org/10.1093/jac/dku021
doi: 10.1093/jac/dku021
pubmed: 24521855
Clinical Laboratory Standards Institute (2008) Reference Method for Broth Dilution Antifungal Susceptibility Testing of Filamentous Fungi-2nd Edition: Approved Standard M38-A2. Wayne, PA, USA CLSI
Pereira DIB, Santurio JM, Alves SH et al (2007) Caspofungin in vitro and in vivo activity against Brazilian Pythium insidiosum strains isolated from animals. J Antimicrob Chemother 60:1168–1171. https://doi.org/10.1093/jac/dkm332
doi: 10.1093/jac/dkm332
pubmed: 17785281
Fonseca AOS, Botton SA, Nogueira CEW et al (2014) In vitro reproduction of the life cycle of Pythium insidiosum from kunkers equine and their role in the epidemiology of pythiosis. Mycopathologia 177:123–127. https://doi.org/10.1007/s11046-013-9720-6
doi: 10.1007/s11046-013-9720-6
pubmed: 24326464
Valente JSS, Braga CQ, Brasil CL et al (2019) In vitro anti-Pythium insidiosum activity of biogenic silver nanoparticles. Med Mycol 57:858–863. https://doi.org/10.1093/mmy/myy147
doi: 10.1093/mmy/myy147
Moureu S, Violleau F, Ali Haimoud-Lekhal D et al (2015) Ozonation of sunflower oils: Impact of experimental conditions on the composition and the antibacterial activity of ozonized oils. Chem Phys Lipids 186:79–85. https://doi.org/10.1016/j.chemphyslip.2015.01.004
doi: 10.1016/j.chemphyslip.2015.01.004
pubmed: 25623845
Díaz MF, Hernández R, Martínez G et al (2006) Comparative study of ozonized olive oil and ozonized sunflower oil. J Braz Chem Soc 17:403–407. https://doi.org/10.1590/S0103-50532006000200026
doi: 10.1590/S0103-50532006000200026
Díaz MF, Sánchez Y, Gómez M et al (2012) Physicochemical characteristics of ozonated sunflower oils obtained by different procedures. Grasas Aceites 63:466–474. https://doi.org/10.3989/gya.073212
doi: 10.3989/gya.073212
Mendoza L, Ajello L, McGinnis MR (1996) Infections caused by the oomycetous pathogen Pythium insidiosum. J Mycol Méd 6:151–164
Di Filippo PA, Ribeiro LMF, Gobbi FP et al (2020) Effects of pure and ozonated sunflower seed oil (Helianthus annuus) on hypergranulation tissue formation, infection and healing of equine lower limb wounds. Braz J Vet Med 42:e113520. https://doi.org/10.29374/2527-2179.bjvm1115321
doi: 10.29374/2527-2179.bjvm1115321
Pietrocola G, Ceci M, Preda F et al (2018) Evaluation of the antibacterial activity of a new ozonized olive oil against oral and periodontal pathogens. J Clin Exp Dent 10:e1103–e1108. https://doi.org/10.4317/jced.54929
doi: 10.4317/jced.54929
pubmed: 30607228
pmcid: 6311406
Xiang Y, Lu J, Li F et al (2018) Bactericidal effect of ozonated camellia oil on Staphylococcus aureus in vitro. Journal of Central South University. Med Sci 43:139–142. https://doi.org/10.11817/j.issn.1672-7347.2018.02.005
doi: 10.11817/j.issn.1672-7347.2018.02.005
Quintana MCF, Domingues IM, Ribeiro AR (2019) Uso de óleo ozonizado no tratamento de mastite subclínica em vaca Jersey: Relato de caso. Pubvet 13:1–4. https://doi.org/10.31533/pubvet.v13n5a336.1-4
doi: 10.31533/pubvet.v13n5a336.1-4
Guerrer LV, Cunha KC, Nogueira MCL et al (2012) “In vitro” antifungal activity of ozonized sunflower oil on yeasts from onychomycosis. Braz J Microbiol 43:1315–1318. https://doi.org/10.1590/S1517-838220120004000011
doi: 10.1590/S1517-838220120004000011
pubmed: 24031958
pmcid: 3769014
Monzillo V, Lallitto F, Russo A et al (2020) Ozonized Gel against four Candida species: A pilot study and clinical perspectives. Materials 13:1731. https://doi.org/10.3390/ma13071731
doi: 10.3390/ma13071731
pubmed: 32276304
pmcid: 7178640
Guimarães NM, De Oliveira IF, Kozusny-Andreani DI (2020) Eficácia de óleos vegetais in natura e ozonizados no controle de Sporothrix schenckii. Int J Dev Res 10:41970–41974. https://doi.org/10.37118/ijdr.20361.11.2020
doi: 10.37118/ijdr.20361.11.2020