Research Note: Transcriptome analysis of skeletal muscles of black-boned chickens, including 2 types (wild and mutated) of Taihe black-boned silky fowl and 1 type (wild) of Yugan black-boned chicken.
GSEA
Taihe black-boned silky fowl
melanin
skeletal muscle
transcriptome
Journal
Poultry science
ISSN: 1525-3171
Titre abrégé: Poult Sci
Pays: England
ID NLM: 0401150
Informations de publication
Date de publication:
27 Oct 2023
27 Oct 2023
Historique:
received:
18
07
2023
revised:
10
10
2023
accepted:
23
10
2023
medline:
25
11
2023
pubmed:
25
11
2023
entrez:
24
11
2023
Statut:
aheadofprint
Résumé
The large amount of melanin deposited in Taihe black-boned silky fowl and other black-boned chicken breeds is a highly valued trait due to its desirable nutritional and functional properties, such as antiaging, immune-enhancing, and antifatigue properties. To identify the candidate genes and pathways potentially responsible for melanogenesis in Taihe black-boned silky fowl, digital gene expression tag (DGE-tag)-based transcriptome analyses were performed for 2 groups: wild-type Taihe black-boned silky fowl (TH-1245) vs. mutated Taihe black-boned silky fowl (BY-1245) and TH-1245 vs. wild-type Yugan black-boned chicken (YG-1245). In total, 430 and 765 differentially expressed genes (DEGs) were identified and 13 DEGs displaying different gene expression patterns between the 2 groups were considered valuable for further investigation, such as ANKRD1, MYOZ2, and MYOD1. Furthermore, 6 functionally grouped networks composed of 36 significant GO terms, mainly involved in muscle-related and signaling-related biological processes, were screened by functional enrichment network analysis. In addition, protein-protein interaction (PPI) network analysis identifies 2 top clusters containing 20 hub genes for 2 comparison groups. MYL1 and RPS14 were considered the most potential candidate genes among all hub genes. The Gene Set Enrichment Analysis (GSEA) results showed that the terms and pathways, such as muscle system process, arachidonic acid metabolism, melanogenesis, and tyrosine metabolism, may play important roles in the melanogenesis and further investigations were needed to clarify the relationships between these pathways and melanin. Overall, these results are helpful for furthering our understanding of melanogenesis in breast muscle of Taihe black-boned silky fowl and Yugan black-boned chicken.
Identifiants
pubmed: 38000345
pii: S0032-5791(23)00759-9
doi: 10.1016/j.psj.2023.103240
pmc: PMC10701445
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
103240Informations de copyright
Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.
Références
Genome Res. 2011 Nov;21(11):1905-15
pubmed: 21844123
J Anim Sci Biotechnol. 2021 May 5;12(1):55
pubmed: 33952351
PLoS One. 2015 Jun 01;10(6):e0127301
pubmed: 26030885
Int J Mol Sci. 2021 Nov 22;22(22):
pubmed: 34830490
Cell Physiol Biochem. 2018;46(6):2373-2384
pubmed: 29742500
Poult Sci. 2018 Oct 1;97(10):3446-3455
pubmed: 29982752
Br Poult Sci. 2023 Feb;64(1):19-25
pubmed: 35979716
Theory Biosci. 2012 Dec;131(4):281-5
pubmed: 22872506
Anim Biotechnol. 2019 Jul;30(3):233-241
pubmed: 30601081
Sci Rep. 2020 Jul 21;10(1):12088
pubmed: 32694523
BMC Genomics. 2020 Jul 23;21(1):511
pubmed: 32703156
Genes (Basel). 2022 Oct 29;13(11):
pubmed: 36360215