Karyotypes of water frogs from the Pelophylax esculentus complex: results of cross-species chromosomal painting.
Chromosomal painting
Chromosome evolution
Clonal hybrids
Cytogenetics
Fluorescence in situ hybridization
Pelophylax esculentus complex
Journal
Chromosoma
ISSN: 1432-0886
Titre abrégé: Chromosoma
Pays: Austria
ID NLM: 2985138R
Informations de publication
Date de publication:
25 Nov 2023
25 Nov 2023
Historique:
received:
08
08
2023
accepted:
07
11
2023
revised:
01
11
2023
medline:
25
11
2023
pubmed:
25
11
2023
entrez:
24
11
2023
Statut:
aheadofprint
Résumé
Amphibian species have the largest genome size enriched with repetitive sequences and relatively similar karyotypes. Moreover, many amphibian species frequently hybridize causing nuclear and mitochondrial genome introgressions. In addition, hybridization in some amphibian species may lead to clonality and polyploidization. All such events were found in water frogs from the genus Pelophylax. Among the species within the genus Pelophylax, P. esculentus complex is the most widely distributed and well-studied. This complex includes two parental species, P. ridibundus and P. lessonae, and their hybrids, P. esculentus, reproducing hemiclonally. Parental species and their hybrids have similar but slightly polymorphic karyotypes, so their precise identification is still required. Here, we have developed a complete set of 13 chromosome painting probes for two parental species allowing the precise identification of all chromosomes. Applying chromosomal painting, we identified homologous chromosomes in both parental species and orthologous chromosomes in their diploid hemiclonal hybrids. Comparative painting did not reveal interchromosomal exchanges between the studied water frog species and their hybrids. Using cross-specific chromosome painting, we detected unequal distribution of the signals along chromosomes suggesting the presence of species-specific tandem repeats. Application of chromosomal paints to the karyotypes of hybrids revealed differences in the intensity of staining for P. ridibundus and P. lessonae chromosomes. Thus, both parental genomes have a divergence in unique sequences. Obtained chromosome probes may serve as a powerful tool to unravel chromosomal evolution in phylogenetically related species, identify individual chromosomes in different cell types, and investigate the elimination of chromosomes in hybrid water frogs.
Identifiants
pubmed: 38001396
doi: 10.1007/s00412-023-00812-8
pii: 10.1007/s00412-023-00812-8
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Russian Science Foundation
ID : 20-74-00030
Organisme : RVO
ID : 67985904
Organisme : Grantová Agentura České Republiky
ID : 23-07028K
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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