Factors affecting the number of bacteria in saliva and oral care methods for the recovery of bacteria in contaminated saliva after brushing: a randomized controlled trial.
Bacterial counts
Brushing
Mouthwash
Oral care
Journal
BMC oral health
ISSN: 1472-6831
Titre abrégé: BMC Oral Health
Pays: England
ID NLM: 101088684
Informations de publication
Date de publication:
24 Nov 2023
24 Nov 2023
Historique:
received:
24
07
2023
accepted:
18
11
2023
medline:
27
11
2023
pubmed:
25
11
2023
entrez:
24
11
2023
Statut:
epublish
Résumé
Oral care is important in preventing aspiration pneumonia in older adults. However, it is not clear what kind of oral care can reduce the number of bacteria in saliva. The purposes of this study are to clarify whether there is a relationship between plaque amounts and salivary bacterial counts, and how bacteria dispersed into the oral cavity by brushing can be reduced. First, saliva samples were collected from 10 healthy adult volunteers after 30 h of unbrushing and after thorough brushing, and the total bacterial count was determined by real-time PCR. Next, 40 older adults attending an outpatient dental clinic were randomly assigned into two groups: a wiping group (20 patients) and a mouthwashing group (20 patients). Saliva was collected before and after brushing, and after wiping in the wiping group and after mouthwashing in the mouthwashing group, and the total bacterial count was quantified by real-time PCR. In a study of volunteers, there was no association between plaque amounts and salivary bacterial counts. In a study of older adult patients, salivary bacterial counts were significantly higher in patients with higher oral hygiene index and fewer remaining teeth. Brushing increased salivary bacterial counts. Wiping did not significantly reduce the number of bacteria, while mouthwash returned the increased number of bacteria after brushing to the pre-brushing level. There is no direct relationship between the amount of plaque and the number of bacteria in saliva. Brushing disperses bacteria into the oral cavity, resulting in a marked increase in the number of bacteria in saliva. Wiping does not collect the dispersed bacteria, and it seems essential to rinse the mouth after brushing. UMIN000045854.
Sections du résumé
BACKGROUND
BACKGROUND
Oral care is important in preventing aspiration pneumonia in older adults. However, it is not clear what kind of oral care can reduce the number of bacteria in saliva. The purposes of this study are to clarify whether there is a relationship between plaque amounts and salivary bacterial counts, and how bacteria dispersed into the oral cavity by brushing can be reduced.
METHODS
METHODS
First, saliva samples were collected from 10 healthy adult volunteers after 30 h of unbrushing and after thorough brushing, and the total bacterial count was determined by real-time PCR. Next, 40 older adults attending an outpatient dental clinic were randomly assigned into two groups: a wiping group (20 patients) and a mouthwashing group (20 patients). Saliva was collected before and after brushing, and after wiping in the wiping group and after mouthwashing in the mouthwashing group, and the total bacterial count was quantified by real-time PCR.
RESULTS
RESULTS
In a study of volunteers, there was no association between plaque amounts and salivary bacterial counts. In a study of older adult patients, salivary bacterial counts were significantly higher in patients with higher oral hygiene index and fewer remaining teeth. Brushing increased salivary bacterial counts. Wiping did not significantly reduce the number of bacteria, while mouthwash returned the increased number of bacteria after brushing to the pre-brushing level.
CONCLUSIONS
CONCLUSIONS
There is no direct relationship between the amount of plaque and the number of bacteria in saliva. Brushing disperses bacteria into the oral cavity, resulting in a marked increase in the number of bacteria in saliva. Wiping does not collect the dispersed bacteria, and it seems essential to rinse the mouth after brushing.
TRIAL REGISTRATION
BACKGROUND
UMIN000045854.
Identifiants
pubmed: 38001433
doi: 10.1186/s12903-023-03676-7
pii: 10.1186/s12903-023-03676-7
pmc: PMC10675882
doi:
Substances chimiques
Mouthwashes
0
Types de publication
Randomized Controlled Trial
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
917Subventions
Organisme : J Grants-in-Aid for Scientific Research
ID : JP22K10295
Informations de copyright
© 2023. The Author(s).
Références
Int J Environ Res Public Health. 2022 Jun 21;19(13):
pubmed: 35805211
J Oral Rehabil. 2007 Jun;34(6):442-7
pubmed: 17518979
Gerontology. 2023;69(3):282-288
pubmed: 36202077
Cochrane Database Syst Rev. 2016 Oct 25;10:CD008367
pubmed: 27778318
Am Rev Respir Dis. 1993 Aug;148(2):352-7
pubmed: 8342898
BMC Oral Health. 2020 Feb 24;20(1):62
pubmed: 32093667
Trials. 2013 May 07;14:126
pubmed: 23782628
Semin Respir Infect. 1987 Mar;2(1):20-33
pubmed: 3321264
Am J Med. 2002 Feb 15;112(3):219-25
pubmed: 11893349
J Speech Lang Hear Res. 2021 Feb 17;64(2):464-480
pubmed: 33405973
Gerontology. 2021;67(5):517-524
pubmed: 33596585
J Am Dent Assoc. 1964 Jan;68:7-13
pubmed: 14076341
BMC Oral Health. 2016 Jun 07;16(1):67
pubmed: 27268137
Gerodontology. 2004 Jun;21(2):108-11
pubmed: 15185991
AJR Am J Roentgenol. 1994 Dec;163(6):1333-7
pubmed: 7992723
Cochrane Database Syst Rev. 2022 Nov 16;11:CD012416
pubmed: 36383760
Gerodontology. 2013 Mar;30(1):3-9
pubmed: 22390255
Ann Intern Med. 1995 Feb 1;122(3):179-86
pubmed: 7810935
BMJ Open. 2022 Jun 15;12(6):e061893
pubmed: 35705350