The Effect of Ficin Immobilized on Carboxymethyl Chitosan on Biofilms of Oral Pathogens.
bacterial biofilms
carboxymethyl chitosan
ficin
oral microbiota
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
08 Nov 2023
08 Nov 2023
Historique:
received:
13
10
2023
revised:
31
10
2023
accepted:
07
11
2023
medline:
27
11
2023
pubmed:
25
11
2023
entrez:
25
11
2023
Statut:
epublish
Résumé
In the last decade, Ficin, a proteolytic enzyme extracted from the latex sap of the wild fig tree, has been widely investigated as a promising tool for the treatment of microbial biofilms, wound healing, and oral care. Here we report the antibiofilm properties of the enzyme immobilized on soluble carboxymethyl chitosan (CMCh) and CMCh itself. Ficin was immobilized on CMCh with molecular weights of either 200, 350 or 600 kDa. Among them, the carrier with a molecular weight of 200 kDa bound the maximum amount of enzyme, binding up to 49% of the total protein compared to 19-32% of the total protein bound to other CMChs. Treatment with pure CMCh led to the destruction of biofilms formed by
Identifiants
pubmed: 38003281
pii: ijms242216090
doi: 10.3390/ijms242216090
pmc: PMC10671066
pii:
doi:
Substances chimiques
Ficain
EC 3.4.22.3
Chlorhexidine
R4KO0DY52L
Chitosan
9012-76-4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Russian Science Foundation
ID : RSF-21-74-20053
Organisme : Subsidy allocated to Kazan Federal University for the state assignment in the sphere of scientific activities
ID : Project No. FZSM-2022-0017
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