Identification of Host Factors for Rift Valley Fever Phlebovirus.
A549 cells
LACV
MP-12
RVFV
WDR7 gene
bunyavirus
host factor
phlebovirus
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
13 Nov 2023
13 Nov 2023
Historique:
received:
28
09
2023
revised:
26
10
2023
accepted:
30
10
2023
medline:
27
11
2023
pubmed:
25
11
2023
entrez:
25
11
2023
Statut:
epublish
Résumé
Rift Valley fever phlebovirus (RVFV) is a zoonotic pathogen that causes Rift Valley fever (RVF) in livestock and humans. Currently, there is no licensed human vaccine or antiviral drug to control RVF. Although multiple species of animals and humans are vulnerable to RVFV infection, host factors affecting susceptibility are not well understood. To identify the host factors or genes essential for RVFV replication, we conducted CRISPR-Cas9 knockout screening in human A549 cells. We then validated the putative genes using siRNA-mediated knock-downs and CRISPR-Cas9-mediated knock-out studies. The role of a candidate gene in the virus replication cycle was assessed by measuring intracellular viral RNA accumulation, and the virus titers were analyzed using plaque assay or TCID
Identifiants
pubmed: 38005928
pii: v15112251
doi: 10.3390/v15112251
pmc: PMC10675714
pii:
doi:
Substances chimiques
RNA, Small Interfering
0
WDR7 protein, human
0
Adaptor Proteins, Signal Transducing
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIA NIH HHS
ID : P01 AG000001
Pays : United States
Organisme : NIGMS NIH HHS
ID : P20 GM130448
Pays : United States
Commentaires et corrections
Type : UpdateOf
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