Inflammatory related plasma proteins involved in acute preschool wheeze.
asthma
inflammation
olink
plasma proteins
preschool wheeze
Journal
Clinical and translational allergy
ISSN: 2045-7022
Titre abrégé: Clin Transl Allergy
Pays: England
ID NLM: 101576043
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
revised:
01
10
2023
received:
19
05
2023
accepted:
16
10
2023
medline:
26
11
2023
pubmed:
26
11
2023
entrez:
25
11
2023
Statut:
ppublish
Résumé
Preschool wheeze is a risk factor for asthma development. However, the molecular mechanism behind a wheezing episode is not well understood. Our aims were to assess the association of plasma proteins with acute preschool wheeze and to study the proteins with differential expression at the acute phase at revisit after 3 months. Additionally, to investigate the relationship between protein expression and clinical parameters. We measured 92 inflammatory proteins in plasma and clinical parameters from 145 children during an episode of preschool wheeze (PW) and at the revisit after 3 months (PW-R, n = 113/145) and 101 healthy controls (HC) aged 6-48 months in the GEWAC cohort using the antibody-mediated proximity extension-based assay (Olink Proteomics, Uppsala). Of the 74 analysed proteins, 52 were differentially expressed between PW and HC. The expression profiles of the top 10 proteins, Oncostatin M (OSM), IL-10, IL-6, Fibroblast growth factor 21 (FGF21), AXIN1, CXCL10, SIRT2, TNFSF11, Tumour necrosis factor β (TNF-β) and CASP8, could almost entirely separate PW from HC. Five out of 10 proteins were associated with intake of oral corticosteroids (OCS) 24 h preceding blood sampling (OSM, CASP8, IL-10, TNF-β and CXCL10). No differences in protein expression were seen between PWs with or without OCS in comparison to HC. At the revisit after 3 months, differential protein expressions were still seen between PW-R and HC for three (IL-10, SIRT2 and FGF21) of the 10 proteins. Our results contribute to unravelling potential immunopathological pathways shared between preschool wheeze and asthma.
Sections du résumé
BACKGROUND
BACKGROUND
Preschool wheeze is a risk factor for asthma development. However, the molecular mechanism behind a wheezing episode is not well understood.
OBJECTIVE
OBJECTIVE
Our aims were to assess the association of plasma proteins with acute preschool wheeze and to study the proteins with differential expression at the acute phase at revisit after 3 months. Additionally, to investigate the relationship between protein expression and clinical parameters.
METHOD
METHODS
We measured 92 inflammatory proteins in plasma and clinical parameters from 145 children during an episode of preschool wheeze (PW) and at the revisit after 3 months (PW-R, n = 113/145) and 101 healthy controls (HC) aged 6-48 months in the GEWAC cohort using the antibody-mediated proximity extension-based assay (Olink Proteomics, Uppsala).
RESULTS
RESULTS
Of the 74 analysed proteins, 52 were differentially expressed between PW and HC. The expression profiles of the top 10 proteins, Oncostatin M (OSM), IL-10, IL-6, Fibroblast growth factor 21 (FGF21), AXIN1, CXCL10, SIRT2, TNFSF11, Tumour necrosis factor β (TNF-β) and CASP8, could almost entirely separate PW from HC. Five out of 10 proteins were associated with intake of oral corticosteroids (OCS) 24 h preceding blood sampling (OSM, CASP8, IL-10, TNF-β and CXCL10). No differences in protein expression were seen between PWs with or without OCS in comparison to HC. At the revisit after 3 months, differential protein expressions were still seen between PW-R and HC for three (IL-10, SIRT2 and FGF21) of the 10 proteins.
CONCLUSION
CONCLUSIONS
Our results contribute to unravelling potential immunopathological pathways shared between preschool wheeze and asthma.
Identifiants
pubmed: 38006384
doi: 10.1002/clt2.12308
pmc: PMC10618892
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e12308Subventions
Organisme : the Samaritan Foundation
Organisme : Region Stockholm
Organisme : Stiftelsen Konung Gustaf V:s 80-årsfond
Organisme : The Swedish Asthma and Allergy Association's Research Foundation
Organisme : Freemason Child House Foundation in Stockholm
Organisme : the Centre for Allergy Research at Karolinska Institutet
Organisme : Karolinska Institutet
Organisme : H.K.H. Kronprinsessan Lovisas Förening för Barnasjukvård
Organisme : the Hesselman Foundation
Organisme : The Swedish Research Council
Organisme : Axel Tielmans Memorial Fund
Organisme : the Swedish Heart-Lung Foundation
Organisme : Sigurd and Elsa Goljes Memorial Fund
Organisme : Konsul Th C Berghs Stiftelse
Organisme : the Stockholm County Council
Organisme : Insamlingsstiftelsen Cancer- och Allergifonden
Informations de copyright
© 2023 The Authors. Clinical and Translational Allergy published by John Wiley & Sons Ltd on behalf of European Academy of Allergy and Clinical Immunology.
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