SGC-CLK-1: A chemical probe for the Cdc2-like kinases CLK1, CLK2, and CLK4.

CLK1 CLK2 CLK4 Chemical probe Splicing

Journal

Current research in chemical biology
ISSN: 2666-2469
Titre abrégé: Curr Res Chem Biol
Pays: Netherlands
ID NLM: 9918434484806676

Informations de publication

Date de publication:
2023
Historique:
medline: 27 11 2023
pubmed: 27 11 2023
entrez: 27 11 2023
Statut: ppublish

Résumé

Small molecule modulators are important tools to study both basic biology and the complex signaling of protein kinases. The cdc2-like kinases (CLK) are a family of four kinases that have garnered recent interest for their involvement in a diverse set of diseases such as neurodegeneration, autoimmunity, and many cancers. Targeted medicinal chemistry around a CLK inhibitor hit identified through screening of a kinase inhibitor set against a large panel of kinases allowed us to identify a potent and selective inhibitor of CLK1, 2, and 4. Here, we present the synthesis, selectivity, and preliminary biological characterization of this compound - SGC-CLK-1 (CAF-170). We further show CLK2 has the highest binding affinity, and high CLK2 expression correlates with a lower IC

Identifiants

pubmed: 38009092
doi: 10.1016/j.crchbi.2023.100045
pmc: PMC10673624
mid: NIHMS1942723
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : NCI NIH HHS
ID : K99 CA279896
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS115403
Pays : United States
Organisme : NCI NIH HHS
ID : K00 CA234799
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS125318
Pays : United States
Organisme : NCATS NIH HHS
ID : R44 TR001916
Pays : United States
Organisme : NINDS NIH HHS
ID : R21 NS122375
Pays : United States
Organisme : NIDDK NIH HHS
ID : U24 DK116204
Pays : United States
Organisme : NINDS NIH HHS
ID : R21 NS126810
Pays : United States

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Auteurs

Deanna Tiek (D)

The Ken & Ruth Davee Department of Neurology, The Lou and Jean Malnati Brain Tumor Institute, The Robert H. Lurie Comprehensive Cancer Center, Simpson Querrey Institute for Epigenetics, Northwestern University Feinberg School of Medicine, Chicago, IL, 60611, USA.

Carrow I Wells (CI)

Structural Genomics Consortium, UNC Eshelman School of Pharmacy, The University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA.

Martin Schröder (M)

Structural Genomics Consortium (SGC), Buchmann Institute for Life Sciences (BMLS), Goethe University Frankfurt am Main, Max-von-Laue-Str. 15, 60438, Frankfurt am Main, Germany.
Institut für Pharmazeutische Chemie, Goethe University Frankfurt am Main, Max-von-Laue-Str. 9, Frankfurt am Main, 60438, Germany.

Xiao Song (X)

The Ken & Ruth Davee Department of Neurology, The Lou and Jean Malnati Brain Tumor Institute, The Robert H. Lurie Comprehensive Cancer Center, Simpson Querrey Institute for Epigenetics, Northwestern University Feinberg School of Medicine, Chicago, IL, 60611, USA.

Carla Alamillo-Ferrer (C)

Structural Genomics Consortium, UNC Eshelman School of Pharmacy, The University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA.

Anshika Goenka (A)

The Ken & Ruth Davee Department of Neurology, The Lou and Jean Malnati Brain Tumor Institute, The Robert H. Lurie Comprehensive Cancer Center, Simpson Querrey Institute for Epigenetics, Northwestern University Feinberg School of Medicine, Chicago, IL, 60611, USA.

Rebeca Iglesia (R)

The Ken & Ruth Davee Department of Neurology, The Lou and Jean Malnati Brain Tumor Institute, The Robert H. Lurie Comprehensive Cancer Center, Simpson Querrey Institute for Epigenetics, Northwestern University Feinberg School of Medicine, Chicago, IL, 60611, USA.

Minghui Lu (M)

The Ken & Ruth Davee Department of Neurology, The Lou and Jean Malnati Brain Tumor Institute, The Robert H. Lurie Comprehensive Cancer Center, Simpson Querrey Institute for Epigenetics, Northwestern University Feinberg School of Medicine, Chicago, IL, 60611, USA.

Bo Hu (B)

The Ken & Ruth Davee Department of Neurology, The Lou and Jean Malnati Brain Tumor Institute, The Robert H. Lurie Comprehensive Cancer Center, Simpson Querrey Institute for Epigenetics, Northwestern University Feinberg School of Medicine, Chicago, IL, 60611, USA.

Frank Kwarcinski (F)

Luceome Biotechnologies LLC, Tucson, AZ, 85719, USA.

Parvathi Sintha (P)

Luceome Biotechnologies LLC, Tucson, AZ, 85719, USA.

Chandi de Silva (C)

Luceome Biotechnologies LLC, Tucson, AZ, 85719, USA.

Mohammad Anwar Hossain (MA)

Structural Genomics Consortium, UNC Eshelman School of Pharmacy, The University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA.

Alfredo Picado (A)

Structural Genomics Consortium, UNC Eshelman School of Pharmacy, The University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA.

William Zuercher (W)

Structural Genomics Consortium, UNC Eshelman School of Pharmacy, The University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA.

Reena Zutshi (R)

Luceome Biotechnologies LLC, Tucson, AZ, 85719, USA.

Stefan Knapp (S)

Structural Genomics Consortium (SGC), Buchmann Institute for Life Sciences (BMLS), Goethe University Frankfurt am Main, Max-von-Laue-Str. 15, 60438, Frankfurt am Main, Germany.
Institut für Pharmazeutische Chemie, Goethe University Frankfurt am Main, Max-von-Laue-Str. 9, Frankfurt am Main, 60438, Germany.

Rebecca B Riggins (RB)

Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington DC, 20057, USA.

Shi-Yuan Cheng (SY)

The Ken & Ruth Davee Department of Neurology, The Lou and Jean Malnati Brain Tumor Institute, The Robert H. Lurie Comprehensive Cancer Center, Simpson Querrey Institute for Epigenetics, Northwestern University Feinberg School of Medicine, Chicago, IL, 60611, USA.

David H Drewry (DH)

Structural Genomics Consortium, UNC Eshelman School of Pharmacy, The University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA.
UNC Lineberger Comprehensive Cancer Center, School of Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA.

Classifications MeSH