An integrated liver, hippocampus and serum metabolomics based on UPLC-Q-TOF-MS revealed the therapeutical mechanism of Ziziphi Spinosae Semen in p-chlorophenylalanine-induced insomnia rats.
Ziziphi Spinosae Semen
hippocampus
insomnia
liver
metabolomics
Journal
Biomedical chromatography : BMC
ISSN: 1099-0801
Titre abrégé: Biomed Chromatogr
Pays: England
ID NLM: 8610241
Informations de publication
Date de publication:
27 Nov 2023
27 Nov 2023
Historique:
revised:
07
11
2023
received:
07
10
2023
accepted:
09
11
2023
medline:
27
11
2023
pubmed:
27
11
2023
entrez:
27
11
2023
Statut:
aheadofprint
Résumé
Ziziphi Spinosae Semen (ZSS), a well-known herbal medicine for treating insomnia, is popular in not only China but also in Europe, India and Iran. However, its underlying mechanisms remain unclear. In this work, taking the targeted organs of insomnia, the liver and hippocampus, as the objects, a combination metabolomics based on ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was established to illustrate the abnormality of metabolic characteristics of the liver, hippocampus and serum of p-chlorophenylalanine (PCPA)-induced insomnia rats and to demonstrate the mechanism of ZSS in treating insomnia. The results showed that ZSS could restore the brain cell morphology, decrease the degree of hepatocyte necrosis and regulate the disturbance of neurotransmitters and hormones in insomnia rats. In terms of metabolomics, a total of 33 liver metabolites, 25 hippocampal metabolites and 18 serum metabolites were finally selected as the potential biomarkers and an important pathway of phenylalanine, tyrosine and tryptophan biosynthesis was common in three tissues in PCPA rats. Meanwhile, ZSS significantly reversed the levels of 23 liver metabolites, 15 hippocampal metabolites and 5 serum metabolites. The present study demonstrates the actions of ZSS in treating insomnia by enhancing both cerebral and hepatic functions.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e5796Subventions
Organisme : National Natural Science Foundation of China
ID : 81603289
Organisme : National Natural Science Foundation of China
ID : 81603251
Organisme : Natural Science Foundation of Shanxi Province
ID : 20210302123470
Organisme : Natural Science Foundation of Shanxi Province
ID : 20210302123237
Organisme : Innovation Project for Graduate Students in Shanxi Province
ID : 2022Y163
Organisme : Innovation Project for Graduate Students in Shanxi Province
ID : 2023KY144
Organisme : 2023 Scientific Research Project of Traditional Chinese Medicine
ID : 2023ZYYB061
Informations de copyright
© 2023 John Wiley & Sons, Ltd.
Références
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