Improvement of C-to-U RNA editing using an artificial MS2-APOBEC system.
APOBEC3A
APOBEC3G
MS2 system
RNA editing
Journal
Biotechnology journal
ISSN: 1860-7314
Titre abrégé: Biotechnol J
Pays: Germany
ID NLM: 101265833
Informations de publication
Date de publication:
27 Nov 2023
27 Nov 2023
Historique:
revised:
20
11
2023
received:
03
07
2023
accepted:
22
11
2023
pubmed:
27
11
2023
medline:
27
11
2023
entrez:
27
11
2023
Statut:
aheadofprint
Résumé
RNA cytidine deamination (C-to-U editing) has been achieved using the MS2-apolipoprotein B-editing catalytic polypeptide-like (APOBEC)1 editing system. Here, we fused the cytidine deaminase (CDA) enzymes APOBEC3A and APOBEC3G with the MS2 system and examined their RNA editing efficiencies in transfected HEK 293T cells. Given the single-stranded RNA preferences of APOBEC3A and APOBEC3G, we designed unconventional guide RNAs that induced a loop at the target sequence, allowing the target to form a single-stranded structure. Because APOBEC3A and APOBEC3G have different base preferences (5'-TC and 5'-CC, respectively), we introduced the D317W mutation into APOBEC3G to convert its base preference to that of APOBEC3A. Upon co-transfection with a guide RNA that induced the formation of a 14 nt loop on the target sequence, MS2-fused APOBEC3A and APOBEC3G showed high editing efficiency. While the D317W mutation of APOBEC3G led to a slight improvement in editing efficiency, the difference was not statistically significant. These findings indicate that APOBEC3A and APOBEC3G can induce C-to-U RNA editing when transfected with a loop guide RNA. Moreover, the editing efficiency of APOBEC3G can be enhanced by site-specific mutation to alter the base preference. Overall, our results demonstrate that the MS2 system can fuse and catalyze reactions with different enzymes, suggesting that it holds an even greater potential for RNA editing than is utilized currently.
Identifiants
pubmed: 38010373
doi: 10.1002/biot.202300321
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2300321Subventions
Organisme : Japan Society for the Promotion of Science
ID : 17H02204
Organisme : Japan Society for the Promotion of Science
ID : 18K19288
Organisme : Japan Society for the Promotion of Science
ID : 21H02067
Informations de copyright
© 2023 Wiley-VCH GmbH.
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