Structure determination by cryoEM at 100 keV.
100 keV
TEM
cryoEM
electron microscopy
structure determination
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
05 Dec 2023
05 Dec 2023
Historique:
medline:
27
11
2023
pubmed:
27
11
2023
entrez:
27
11
2023
Statut:
ppublish
Résumé
Electron cryomicroscopy can, in principle, determine the structures of most biological molecules but is currently limited by access, specimen preparation difficulties, and cost. We describe a purpose-built instrument operating at 100 keV-including advances in electron optics, detection, and processing-that makes structure determination fast and simple at a fraction of current costs. The instrument attains its theoretical performance limits, allowing atomic resolution imaging of gold test specimens and biological molecular structure determination in hours. We demonstrate its capabilities by determining the structures of eleven different specimens, ranging in size from 140 kDa to 2 MDa, using a fraction of the data normally required. CryoEM with a microscope designed specifically for high-efficiency, on-the-spot imaging of biological molecules will expand structural biology to a wide range of previously intractable problems.
Identifiants
pubmed: 38011573
doi: 10.1073/pnas.2312905120
pmc: PMC10710074
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2312905120Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UP_1201/15
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UP_A025_1012
Pays : United Kingdom
Déclaration de conflit d'intérêts
Competing interests statement:T.W. and M.M.E.-G. are employees of YPS, Ltd. J.D. was an employee of JEOL, UK during this work. M.M. and C.S.-B. are employees of DECTRIS, Ltd. G.M., R.H., and C.J.R. are inventors on a patent application EP No. 20789961.8 filed by UKRI related to this work.
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