The structural basis for light acclimation in phycobilisome light harvesting systems systems in Porphyridium purpureum.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
27 Nov 2023
27 Nov 2023
Historique:
received:
30
03
2023
accepted:
14
11
2023
medline:
29
11
2023
pubmed:
28
11
2023
entrez:
27
11
2023
Statut:
epublish
Résumé
Photosynthetic organisms adapt to changing light conditions by manipulating their light harvesting complexes. Biophysical, biochemical, physiological and genetic aspects of these processes are studied extensively. The structural basis for these studies is lacking. In this study we address this gap in knowledge by focusing on phycobilisomes (PBS), which are large structures found in cyanobacteria and red algae. In this study we focus on the phycobilisomes (PBS), which are large structures found in cyanobacteria and red algae. Specifically, we examine red algae (Porphyridium purpureum) grown under a low light intensity (LL) and a medium light intensity (ML). Using cryo-electron microscopy, we resolve the structure of ML-PBS and compare it to the LL-PBS structure. The ML-PBS is 13.6 MDa, while the LL-PBS is larger (14.7 MDa). The LL-PBS structure have a higher number of closely coupled chromophore pairs, potentially the source of the red shifted fluorescence emission from LL-PBS. Interestingly, these differences do not significantly affect fluorescence kinetics parameters. This indicates that PBS systems can maintain similar fluorescence quantum yields despite an increase in LL-PBS chromophore numbers. These findings provide a structural basis to the processes by which photosynthetic organisms adapt to changing light conditions.
Identifiants
pubmed: 38012412
doi: 10.1038/s42003-023-05586-4
pii: 10.1038/s42003-023-05586-4
pmc: PMC10682464
doi:
Substances chimiques
Phycobilisomes
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1210Subventions
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : 31861143048
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : 073-2458356
Informations de copyright
© 2023. The Author(s).
Références
Microorganisms. 2022 Aug 03;10(8):
pubmed: 36013980
J Mol Biol. 2012 Jan 13;415(2):406-18
pubmed: 22100448
Photosynth Res. 2021 Jan;147(1):91-106
pubmed: 33280077
Nat Methods. 2013 Jun;10(6):584-90
pubmed: 23644547
Physiol Plant. 2022 Nov;174(6):e13802
pubmed: 36259916
Annu Rev Plant Biol. 2008;59:89-113
pubmed: 18444897
J Phys Chem B. 2013 Sep 26;117(38):11200-8
pubmed: 23534376
Nature. 2020 Mar;579(7797):146-151
pubmed: 32076272
Photosynth Res. 2019 Sep;141(3):343-353
pubmed: 30929163
Mol Cell Biochem. 1977 Dec 29;18(2-3):125-40
pubmed: 415227
Biochim Biophys Acta. 2011 Jul;1807(7):847-53
pubmed: 21496452
Biochim Biophys Acta. 2011 Dec;1807(12):1591-9
pubmed: 21907180
Photosynth Res. 2005;85(1):15-32
pubmed: 15977057
Nat Commun. 2021 Mar 25;12(1):1890
pubmed: 33767155
J Phys Chem B. 2013 Aug 8;117(31):9121-8
pubmed: 23799323
FEBS Lett. 1997 Jun 30;410(2-3):428-32
pubmed: 9237676
Biophys J. 2012 Apr 4;102(7):1692-700
pubmed: 22500770
Photosynth Res. 2017 Feb;131(2):187-202
pubmed: 27623780
Sci Rep. 2021 Jul 13;11(1):14367
pubmed: 34257340
Photosynth Res. 2003;76(1-3):207-15
pubmed: 16228579
Trends Plant Sci. 2020 Jan;25(1):92-104
pubmed: 31679992
J Phys Chem B. 2017 Feb 16;121(6):1240-1247
pubmed: 28121148
Science. 2020 Aug 21;369(6506):
pubmed: 32820091
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
Anal Sci. 2003 Jul;19(7):1001-5
pubmed: 12880082
Plant Physiol. 1989 Nov;91(3):1040-3
pubmed: 16667108
Biochim Biophys Acta. 2007 Jun;1767(6):814-9
pubmed: 17234153
J Phys Chem B. 2017 Oct 5;121(39):9196-9202
pubmed: 28872312
Photosynth Res. 2017 Feb;131(2):173-186
pubmed: 27638320
Proc Natl Acad Sci U S A. 2019 Mar 26;116(13):6457-6462
pubmed: 30846551
Commun Biol. 2022 Jul 22;5(1):727
pubmed: 35869258
Biochim Biophys Acta Bioenerg. 2020 Apr 1;1861(4):148047
pubmed: 31306623
J Bacteriol. 1977 Apr;130(1):82-91
pubmed: 856789
Nucleic Acids Res. 2000 Jan 1;28(1):235-42
pubmed: 10592235
Plant Physiol. 2000 Aug;123(4):1415-26
pubmed: 10938358
Plant Physiol. 2022 Jun 27;189(3):1204-1219
pubmed: 35512089
Mol Microbiol. 2008 Apr;68(2):263-76
pubmed: 18284595
FEBS J. 2023 Jan;290(2):400-411
pubmed: 35993149
Elife. 2021 Sep 13;10:
pubmed: 34515634
Biochim Biophys Acta. 2004 Jul 9;1657(2-3):73-81
pubmed: 15238265
Curr Opin Plant Biol. 2017 Jun;37:18-23
pubmed: 28391048
Nat Chem Biol. 2014 Jul;10(7):492-501
pubmed: 24937067
Annu Rev Phys Chem. 2003;54:57-87
pubmed: 12471171
Annu Rev Plant Biol. 2011;62:515-48
pubmed: 21438681
Int J Mol Sci. 2012 Dec 12;13(12):17019-47
pubmed: 23235328
Trends Plant Sci. 2018 Jun;23(6):497-506
pubmed: 29625851
FEBS J. 2021 Feb;288(3):980-994
pubmed: 32428340
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
pubmed: 20383002
Photosynth Res. 2022 Nov;154(2):113-124
pubmed: 36070061
Annu Rev Biophys Biophys Chem. 1985;14:47-77
pubmed: 3924069
J R Soc Interface. 2022 Nov;19(196):20220580
pubmed: 36448289
Nature. 2023 Apr;616(7955):199-206
pubmed: 36922595
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21
pubmed: 20124702
J Bacteriol. 2005 Mar;187(5):1685-94
pubmed: 15716439
J Struct Biol. 2003 Jun;142(3):334-47
pubmed: 12781660
Ann Rev Mar Sci. 2022 Jan 3;14:213-238
pubmed: 34460315
Phys Chem Chem Phys. 2014 Jun 21;16(23):11245-50
pubmed: 24562323
J Biol Chem. 2022 Apr;298(4):101783
pubmed: 35245502
J Phys Chem B. 2012 Mar 1;116(8):2568-74
pubmed: 22257008
Biochim Biophys Acta. 1958 Jan;27(1):205-6
pubmed: 13510272
Subcell Biochem. 2016;79:111-39
pubmed: 27485220
J Struct Biol. 2012 Dec;180(3):519-30
pubmed: 23000701