Mapping of conserved immunodominant epitope peptides in the outer membrane porin (Omp) L of prominent Enterobacteriaceae pathogens associated with gastrointestinal infections.
B-cell epitopes
Epitope conservancy
Outer membrane porin (Omp) L
Sequence homology
Journal
Journal, genetic engineering & biotechnology
ISSN: 2090-5920
Titre abrégé: J Genet Eng Biotechnol
Pays: Netherlands
ID NLM: 101317150
Informations de publication
Date de publication:
28 Nov 2023
28 Nov 2023
Historique:
received:
09
10
2022
accepted:
14
11
2023
medline:
28
11
2023
pubmed:
28
11
2023
entrez:
27
11
2023
Statut:
epublish
Résumé
Members of Enterobacteriaceae such as Escherichia coli O 157:H7, Salmonella sp., Shigella sp., Klebsiella sp., and Citrobacter freundii are responsible for the outbreak of serious foodborne illness and other mucosal infections across the globe. The outer membrane proteins (OMPs) of Enterobacteriaceae are highly immunogenic in eliciting immune responses against pathogens. Moreover, the OMPs are highly conserved in the Enterobacteriaceae family. Sequence homology in the OMPs will ensure the presence of conserved immunodominant regions with predominant epitopes. The OmpL is such an immunogen that is highly conserved among the Enterobacteriaceae pathogens. In this study, we performed computational analysis on the outer membrane porin (Omp) L of prominent Enterobacteriaceae pathogens. Multiple sequence and structural alignment analysis have revealed that the OmpL protein is highly conserved among the selected Enterobacteriaceae pathogens. This amount of sequence and structural homology uncovered the conserved antibody binding B-cell epitopes in the OmpL protein. The B-cell epitopes predicted in the OmpL of Salmonella typhimurium are highly conserved among the other Enterobacteriaceae pathogens. In conclusion, these conserved B-cell epitopes will vouch for the generation of heterologous humoral immune response in conferring cross protection against the Enterobacteriaceae pathogens and control their outbreaks across the globe.
Sections du résumé
BACKGROUND
BACKGROUND
Members of Enterobacteriaceae such as Escherichia coli O 157:H7, Salmonella sp., Shigella sp., Klebsiella sp., and Citrobacter freundii are responsible for the outbreak of serious foodborne illness and other mucosal infections across the globe. The outer membrane proteins (OMPs) of Enterobacteriaceae are highly immunogenic in eliciting immune responses against pathogens. Moreover, the OMPs are highly conserved in the Enterobacteriaceae family. Sequence homology in the OMPs will ensure the presence of conserved immunodominant regions with predominant epitopes. The OmpL is such an immunogen that is highly conserved among the Enterobacteriaceae pathogens. In this study, we performed computational analysis on the outer membrane porin (Omp) L of prominent Enterobacteriaceae pathogens.
RESULTS
RESULTS
Multiple sequence and structural alignment analysis have revealed that the OmpL protein is highly conserved among the selected Enterobacteriaceae pathogens. This amount of sequence and structural homology uncovered the conserved antibody binding B-cell epitopes in the OmpL protein. The B-cell epitopes predicted in the OmpL of Salmonella typhimurium are highly conserved among the other Enterobacteriaceae pathogens.
CONCLUSION
CONCLUSIONS
In conclusion, these conserved B-cell epitopes will vouch for the generation of heterologous humoral immune response in conferring cross protection against the Enterobacteriaceae pathogens and control their outbreaks across the globe.
Identifiants
pubmed: 38012455
doi: 10.1186/s43141-023-00622-6
pii: 10.1186/s43141-023-00622-6
pmc: PMC10682294
doi:
Types de publication
Journal Article
Langues
eng
Pagination
146Informations de copyright
© 2023. The Author(s).
Références
Arterioscler Thromb Vasc Biol. 2003 Jun 1;23(6):1060-5
pubmed: 12702515
Vaccine. 2022 Apr 20;40(18):2604-2611
pubmed: 35331568
PLoS Comput Biol. 2012;8(12):e1002829
pubmed: 23300419
J Genet Eng Biotechnol. 2022 Jul 11;20(1):100
pubmed: 35821357
Vaccine. 2013 Jun 19;31(28):2930-6
pubmed: 23643894
Nucleic Acids Res. 2017 Jul 3;45(W1):W24-W29
pubmed: 28472356
Semin Perinatol. 1999 Jun;23(3):218-25
pubmed: 10405191
Int J Mol Sci. 2022 Mar 10;23(6):
pubmed: 35328398
J Genet Eng Biotechnol. 2021 May 11;19(1):69
pubmed: 33974183
Appl Microbiol Biotechnol. 2021 Nov;105(21-22):8297-8311
pubmed: 34609523
Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W526-31
pubmed: 15215442
Vet Res Commun. 1999 Mar;23(2):81-90
pubmed: 10359152
Infect Immun. 1976 Feb;13(2):373-81
pubmed: 4375
Med J Armed Forces India. 2016 Dec;72(Suppl 1):S59-S61
pubmed: 28050072
Front Microbiol. 2017 Sep 20;8:1805
pubmed: 28979250
Virulence. 2013 Jul 1;4(5):366-72
pubmed: 23624795
Philos Trans R Soc Lond B Biol Sci. 2015 Oct 5;370(1679):
pubmed: 26370935
Appl Microbiol Biotechnol. 2020 Mar;104(6):2651-2661
pubmed: 31997109