Human placental extract suppresses mast cell activation and induces mast cell apoptosis.
Allergy
Degranulation
Human placental extract
Mast cell
Mast cell activation
Passive cutaneous anaphylaxis
Journal
Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology
ISSN: 1710-1484
Titre abrégé: Allergy Asthma Clin Immunol
Pays: England
ID NLM: 101244313
Informations de publication
Date de publication:
27 Nov 2023
27 Nov 2023
Historique:
received:
01
08
2022
accepted:
18
10
2023
medline:
28
11
2023
pubmed:
28
11
2023
entrez:
28
11
2023
Statut:
epublish
Résumé
Human placental extract (HPE) has been documented to facilitate the healing of certain disorders including allergy. However, the effects of HPE on the functionality of mast cells, a critical cell type in allergic diseases, have not been reported. To investigate the effects of HPE on the regulation of allergy with respect to the biological functions of mast cells, the mast cell line C57 or HMC-1 cells were treated with HPE followed by the assessment of cell proliferation, apoptosis, activation, chemotaxis and phagocytosis. Mouse peritoneal mast cells were also investigated for their responses to induction of apoptosis by HPE in vivo. Furthermore, the effect of HPE on mast cell degranulation was confirmed using the passive cutaneous anaphylaxis (PCA) assay, an acute allergy model. HPE was capable of suppressing mast cell proliferation and inducing mast cell apoptosis. Mast cell degranulation in response to compound 48/80- or anti-DNP IgE and DNP-mediated activation was suppressed. In addition, treatment with HPE compromised the production of cytokines by mast cells and cell chemotaxis. These observations were consistent with the dampened passive cutaneous anaphylaxis (PCA) assay following treatment with HPE. This study revealed a suppressive effect of HPE on overall mast cell activities, suggesting a potential regulatory role of HPE on the alleviation of allergic diseases through mast cells.
Sections du résumé
BACKGROUND
BACKGROUND
Human placental extract (HPE) has been documented to facilitate the healing of certain disorders including allergy. However, the effects of HPE on the functionality of mast cells, a critical cell type in allergic diseases, have not been reported.
METHODS
METHODS
To investigate the effects of HPE on the regulation of allergy with respect to the biological functions of mast cells, the mast cell line C57 or HMC-1 cells were treated with HPE followed by the assessment of cell proliferation, apoptosis, activation, chemotaxis and phagocytosis. Mouse peritoneal mast cells were also investigated for their responses to induction of apoptosis by HPE in vivo. Furthermore, the effect of HPE on mast cell degranulation was confirmed using the passive cutaneous anaphylaxis (PCA) assay, an acute allergy model.
RESULTS
RESULTS
HPE was capable of suppressing mast cell proliferation and inducing mast cell apoptosis. Mast cell degranulation in response to compound 48/80- or anti-DNP IgE and DNP-mediated activation was suppressed. In addition, treatment with HPE compromised the production of cytokines by mast cells and cell chemotaxis. These observations were consistent with the dampened passive cutaneous anaphylaxis (PCA) assay following treatment with HPE.
CONCLUSION
CONCLUSIONS
This study revealed a suppressive effect of HPE on overall mast cell activities, suggesting a potential regulatory role of HPE on the alleviation of allergic diseases through mast cells.
Identifiants
pubmed: 38012745
doi: 10.1186/s13223-023-00850-y
pii: 10.1186/s13223-023-00850-y
pmc: PMC10683163
doi:
Types de publication
Journal Article
Langues
eng
Pagination
98Subventions
Organisme : National Natural Science Foundation of China
ID : 81760294
Organisme : National Natural Science Foundation of China
ID : 82260324
Organisme : National Natural Science Foundation of China
ID : 82201968
Organisme : Guizhou Provincial Innovative Talents Team Program
ID : 2019-5610
Organisme : Health Commission of Guizhou Province
ID : gzwkj2022-064
Organisme : Health Commission of Guizhou Province
ID : gzwkj2022-267
Organisme : Health Commission of Guizhou Province
ID : gzwkj2021-361
Informations de copyright
© 2023. The Author(s).
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