Functional TET2 gene polymorphisms increase the risk of neuroblastoma in Chinese children.
TET2
m5C modification
neuroblastoma
polymorphism
susceptibility
Journal
IUBMB life
ISSN: 1521-6551
Titre abrégé: IUBMB Life
Pays: England
ID NLM: 100888706
Informations de publication
Date de publication:
Apr 2024
Apr 2024
Historique:
received:
18
06
2023
accepted:
09
10
2023
pubmed:
28
11
2023
medline:
28
11
2023
entrez:
28
11
2023
Statut:
ppublish
Résumé
The 5-methylcytosine (m5C) is the key chemical modification in RNAs. As one of the demethylases in m5C, TET2 has been shown as a tumor suppressor. However, the impact of TET2 gene polymorphisms on neuroblastoma has not been elucidated. 402 neuroblastoma patients and 473 controls were genotyped for TET2 gene polymorphisms using the TaqMan method. The impact of TET2 gene polymorphisms on neuroblastoma susceptibility was determined using multivariate logistic regression analysis. We also adopted genotype-tissue expression database to explore the impact of TET2 gene polymorphisms on the expression of host and nearby genes. We used the R2 platform and Sangerbox tool to analyze the relationship between gene expression and neuroblastoma risk and prognosis through non-parametric testing and Kaplan-Meier analysis, respectively. We found the TET2 gene polymorphisms (rs10007915 G > C and rs7670522 A > C) and the combined 2-5 risk genotypes can significantly increase neuroblastoma risk. Stratification analysis showed that these significant associations were more prominent in certain subgroups. TET2 rs10007915 G > C and rs7670522 A > C are significantly associated with reduced expression of TET2 mRNA. Moreover, lower expression of TET2 gene is associated with high risk, MYCN amplification, and poor prognosis of neuroblastoma. The rs10007915 G > C and rs7670522 A > C are significantly related to the increased expression of inorganic pyrophosphatase 2 mRNA, and higher expression of PPA2 gene is associated with high risk, MYCN amplification, and poor prognosis of neuroblastomas. In summary, TET2 rs10007915 G > C and rs7670522 A > C significantly confer neuroblastoma susceptibility, and further research is needed to investigate the underlying mechanisms.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
200-211Subventions
Organisme : Basic and Applied Basic Research Foundation of Guangdong Province
ID : 2021A1515111116
Organisme : Guangzhou Science and Technology Project
ID : 202201020622
Organisme : National Natural Science Foundation of China
ID : 82173593
Informations de copyright
© 2023 International Union of Biochemistry and Molecular Biology.
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