Integration of cell wall synthesis and chromosome segregation during cell division in Caulobacter.
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
05 Feb 2024
05 Feb 2024
Historique:
received:
05
11
2022
revised:
17
10
2023
accepted:
10
11
2023
pmc-release:
28
05
2024
medline:
29
11
2023
pubmed:
28
11
2023
entrez:
28
11
2023
Statut:
ppublish
Résumé
To divide, bacteria must synthesize their peptidoglycan (PG) cell wall, a protective meshwork that maintains cell shape. FtsZ, a tubulin homolog, dynamically assembles into a midcell band, recruiting division proteins, including the PG synthases FtsW and FtsI. FtsWI are activated to synthesize PG and drive constriction at the appropriate time and place. However, their activation pathway remains unresolved. In Caulobacter crescentus, FtsWI activity requires FzlA, an essential FtsZ-binding protein. Through time-lapse imaging and single-molecule tracking of Caulobacter FtsW and FzlA, we demonstrate that FzlA is a limiting constriction activation factor that signals to promote conversion of inactive FtsW to an active, slow-moving state. We find that FzlA interacts with the DNA translocase FtsK and place FtsK genetically in a pathway with FzlA and FtsWI. Misregulation of the FzlA-FtsK-FtsWI pathway leads to heightened DNA damage and cell death. We propose that FzlA integrates the FtsZ ring, chromosome segregation, and PG synthesis to ensure robust and timely constriction during Caulobacter division.
Identifiants
pubmed: 38015166
pii: 276433
doi: 10.1083/jcb.202211026
pmc: PMC10683668
pii:
doi:
Substances chimiques
Bacterial Proteins
0
Peptidoglycan
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM108640
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM136221
Pays : United States
Organisme : NIH HHS
ID : R35GM136221
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM136436
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM144272
Pays : United States
Informations de copyright
© 2023 Mahone et al.
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