Identification and characterization of ATM founder mutation in BRCA-negative breast cancer patients of Arab ethnicity.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
27 Nov 2023
Historique:
received: 04 09 2023
accepted: 23 11 2023
medline: 30 11 2023
pubmed: 29 11 2023
entrez: 28 11 2023
Statut: epublish

Résumé

Breast cancer (BC) is the most prevalent malignancy among women worldwide with germline pathogenic variants/likely pathogenic variants (PVs/LPVs) in BRCA1/2 accounting for a large portion of hereditary cases. Recently, heterozygous PVs/LPVs in the ATM serine/threonine kinase or Ataxia-telangiectasia mutated gene (ATM) has been identified as a moderate susceptibility factor for BC in diverse ethnicities. However, the prevalence of ATM PVs/LPVs in BC susceptibility in Arab populations remains largely unexplored. This study investigated the prevalence of ATM PVs/LPVs among BC patients from Saudi Arabia, employing capture-sequencing technology for ATM PVs/LPVs screening in a cohort of 715 unselected BC patients without BRCA1/2 PVs/LPVs. In addition, founder mutation analysis was conducted using the PHASE program. In our entire cohort, four unique PVs/LPVs in the ATM gene were identified in six cases (0.8%). Notably, one recurrent LPV, c.6115G > A:p.Glu2039Lys was identified in three cases, for which haplotype analysis confirmed as a novel putative founder mutation traced back to 13 generations on average. This founder mutation accounted for half of all identified mutant cases and 0.4% of total screened cases. This study further reveals a significant correlation between the presence of ATM mutation and family history of BC (p = 0.0127). These findings underscore an approximate 0.8% prevalence of ATM germline PVs/LPVs in Arab BC patients without BRCA1/2 PVs/LPVs and suggest a founder effect of specific recurrent ATM mutation. These insights can help in the design of a genetic testing strategy tailored to the local population in Saudi Arabia, thereby, enabling more accurate clinical management and risk prediction.

Identifiants

pubmed: 38017116
doi: 10.1038/s41598-023-48231-0
pii: 10.1038/s41598-023-48231-0
pmc: PMC10684510
doi:

Substances chimiques

BRCA1 protein, human 0
BRCA1 Protein 0
BRCA2 protein, human 0
BRCA2 Protein 0
ATM protein, human EC 2.7.11.1
Ataxia Telangiectasia Mutated Proteins EC 2.7.11.1

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

20924

Informations de copyright

© 2023. The Author(s).

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Auteurs

Rong Bu (R)

Human Cancer Genomic Research, Research Center, King Faisal Specialist Hospital and Research Center, 11211, Riyadh, Saudi Arabia.

Abdul K Siraj (AK)

Human Cancer Genomic Research, Research Center, King Faisal Specialist Hospital and Research Center, 11211, Riyadh, Saudi Arabia.

Maha Al-Rasheed (M)

Human Cancer Genomic Research, Research Center, King Faisal Specialist Hospital and Research Center, 11211, Riyadh, Saudi Arabia.

Kaleem Iqbal (K)

Human Cancer Genomic Research, Research Center, King Faisal Specialist Hospital and Research Center, 11211, Riyadh, Saudi Arabia.

Saud Azam (S)

Human Cancer Genomic Research, Research Center, King Faisal Specialist Hospital and Research Center, 11211, Riyadh, Saudi Arabia.

Zeeshan Qadri (Z)

Human Cancer Genomic Research, Research Center, King Faisal Specialist Hospital and Research Center, 11211, Riyadh, Saudi Arabia.

Wael Haqawi (W)

Human Cancer Genomic Research, Research Center, King Faisal Specialist Hospital and Research Center, 11211, Riyadh, Saudi Arabia.

Asma Tulbah (A)

Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital and Research Center, 11211, Riyadh, Saudi Arabia.

Fouad Al-Dayel (F)

Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital and Research Center, 11211, Riyadh, Saudi Arabia.

Osama Almalik (O)

Department of Surgery, King Faisal Specialist Hospital and Research Center, 11211, Riyadh, Saudi Arabia.

Khawla S Al-Kuraya (KS)

Human Cancer Genomic Research, Research Center, King Faisal Specialist Hospital and Research Center, 11211, Riyadh, Saudi Arabia. kkuraya@kfshrc.edu.sa.
Research Centre at KFNCCC, Human Cancer Genomic Research, King Faisal Specialist Hospital and Research Center, MBC#98-16, P.O. Box 3354, 11211, Riyadh, Saudi Arabia. kkuraya@kfshrc.edu.sa.

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