Changing the phospholipid composition of lipid droplets alters localization of select lipid droplet proteins.
Journal
microPublication biology
ISSN: 2578-9430
Titre abrégé: MicroPubl Biol
Pays: United States
ID NLM: 101759238
Informations de publication
Date de publication:
2023
2023
Historique:
received:
17
08
2023
revised:
30
10
2023
accepted:
31
10
2023
medline:
29
11
2023
pubmed:
29
11
2023
entrez:
29
11
2023
Statut:
epublish
Résumé
Our experiments aim to determine if decreasing the amount of phosphatidylcholine (PC) relative to phosphatidylethanolamine (PE) at the lipid droplet surface changes the localization of specific lipid droplet proteins. We manipulate lipid droplet phospholipids in both a cultured mouse hepatocyte (AML12) cell line and on synthetic lipid droplets. Decreasing the PC:PE ratio increases perilipin 2, decreases DGAT2, and does not change rab18 or lanosterol synthase levels on lipid droplets. These differences may be explained by the distinct structural motifs that mediate the protein-lipid droplet interactions.
Identifiants
pubmed: 38021172
doi: 10.17912/micropub.biology.000960
pmc: PMC10656625
doi:
Types de publication
Journal Article
Langues
eng
Informations de copyright
Copyright: © 2023 by the authors.
Déclaration de conflit d'intérêts
The authors declare that there are no conflicts of interest present.
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