Use of 16s rRNA Gene Sequencing for the Identification of Viridans Group Streptococci.
16s rrna pcr
biotyping
gene sequencing
infective endocarditis
viridans group streptococci
Journal
Cureus
ISSN: 2168-8184
Titre abrégé: Cureus
Pays: United States
ID NLM: 101596737
Informations de publication
Date de publication:
Oct 2023
Oct 2023
Historique:
received:
19
09
2023
accepted:
16
10
2023
medline:
29
11
2023
pubmed:
29
11
2023
entrez:
29
11
2023
Statut:
epublish
Résumé
Streptococci belonging to the viridans group are gram-positive bacteria residing as commensals in the upper respiratory, gastrointestinal, and genital tracts in humans. Though they are largely known to be commensals, they may also cause life-threatening infections like infective endocarditis, septicemia, pyogenic infections, pneumonia, and meningitis. The viridans group streptococci (VGS) are usually identified by biotyping; however, species discrimination is not always possible by phenotypic characterization. We identified 53 isolates from blood cultures of patients with infective endocarditis and compared the results of biotyping with 16s rRNA gene sequencing for species identification. Organisms belonging to the mitis group were the most common. 16s rRNA gene polymerase chain reaction and sequencing were useful in identifying the etiological agents at the species level.
Identifiants
pubmed: 38022358
doi: 10.7759/cureus.47125
pmc: PMC10648447
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e47125Informations de copyright
Copyright © 2023, Menon et al.
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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