Sterility testing of germ-free mouse colonies.

Animals Mice Sterilization Germ-Free Life Biomedical Research Infertility
contaminant culture germ-free gnotobiology isolator microbiota sterility

Journal

Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960

Informations de publication

Date de publication:
2023
Historique:
received: 09 08 2023
accepted: 28 09 2023
medline: 1 12 2023
pubmed: 29 11 2023
entrez: 29 11 2023
Statut: epublish

Résumé

In biomedical research, germ-free and gnotobiotic mouse models enable the mechanistic investigation of microbiota-host interactions and their role on (patho)physiology. Throughout any gnotobiotic experiment, standardized and periodic microbiological testing of defined gnotobiotic housing conditions is a key requirement. Here, we review basic principles of germ-free isolator technology, the suitability of various sterilization methods, and the use of sterility testing methods to monitor germ-free mouse colonies. We also discuss their effectiveness and limitations, and share the experience with protocols used in our facility. In addition, possible sources of isolator contamination are discussed and an overview of reported contaminants is provided.

Identifiants

DOI: 10.3389/fimmu.2023.1275109 PMID: 38022683 PMC: PMC10662041
pubmed: 38022683
doi: 10.3389/fimmu.2023.1275109
pmc: PMC10662041
doi:

Types de publication

Journal Article Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

1275109

Informations de copyright

Copyright © 2023 Dremova, Mimmler, Paeslack, Khuu, Gao, Bosmann, Garo, Schön, Mechler, Beneich, Rebling, Mann, Pontarollo, Kiouptsi and Reinhardt.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Olga Dremova (O)

Center for Thrombosis and Hemostasis (CTH), University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.

Maximilian Mimmler (M)

Center for Thrombosis and Hemostasis (CTH), University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.

Nadja Paeslack (N)

Center for Thrombosis and Hemostasis (CTH), University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.

My Phung Khuu (MP)

Center for Thrombosis and Hemostasis (CTH), University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.

Zhenling Gao (Z)

Center for Thrombosis and Hemostasis (CTH), University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.

Markus Bosmann (M)

Center for Thrombosis and Hemostasis (CTH), University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
Pulmonary Center, Department of Medicine, Boston University School of Medicine, Boston, MA, United States.

Lucien P Garo (LP)

Pulmonary Center, Department of Medicine, Boston University School of Medicine, Boston, MA, United States.

Nathalie Schön (N)

Center for Thrombosis and Hemostasis (CTH), University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.

Alexa Mechler (A)

Center for Thrombosis and Hemostasis (CTH), University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.

Yunes Beneich (Y)

Center for Thrombosis and Hemostasis (CTH), University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.

Vivian Rebling (V)

Center for Thrombosis and Hemostasis (CTH), University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.

Amrit Mann (A)

Center for Thrombosis and Hemostasis (CTH), University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.

Giulia Pontarollo (G)

Center for Thrombosis and Hemostasis (CTH), University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.

Klytaimnistra Kiouptsi (K)

Center for Thrombosis and Hemostasis (CTH), University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
German Center for Cardiovascular Research (DZHK), University Medical Center of the Johannes Gutenberg-University Mainz, Partner Site Rhine-Main, Mainz, Germany.

Christoph Reinhardt (C)

Center for Thrombosis and Hemostasis (CTH), University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
German Center for Cardiovascular Research (DZHK), University Medical Center of the Johannes Gutenberg-University Mainz, Partner Site Rhine-Main, Mainz, Germany.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Sterility testing of germ-free mouse colonies.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Sterility testing of germ-free mouse colonies.
questionsmedicales.fr › Environnement et santé publique › Santé publique › Pratiques en santé publique › Contrôle des maladies transmissibles › Prévention des infections › Stérilisation › Sterility testing of germ-free mouse colonies.
questionsmedicales.fr › Phénomènes microbiologiques › Axénie › Sterility testing of germ-free mouse colonies.
questionsmedicales.fr › Disciplines des sciences naturelles › Science › Recherche › Recherche biomédicale › Sterility testing of germ-free mouse colonies.
questionsmedicales.fr › Maladies urogénitales › Maladies génitales › Infertilité › Sterility testing of germ-free mouse colonies.