Expression and Biological Evaluation of an Engineered Recombinant L-asparaginase Designed by
L-asparaginase
Protein design
Recombinant protein
Site-directed mutagenesis
Journal
Advanced pharmaceutical bulletin
ISSN: 2228-5881
Titre abrégé: Adv Pharm Bull
Pays: Iran
ID NLM: 101578021
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
received:
16
01
2023
revised:
31
05
2023
accepted:
10
06
2023
medline:
29
11
2023
pubmed:
29
11
2023
entrez:
29
11
2023
Statut:
ppublish
Résumé
Medical usage of L-asparaginase (ASNase), the first-line of acute lymphoblastic leukemia treatment, is linked to allergic responses and toxicities, which necessitates the development of new bio-better ASNases. The aim of the current study was Based on 21 experimentally reported mutations for ASNase, a virtual library of mutated enzymes with all 7546 possible combinations of up to 4 mutations was generated. Three-dimensional models of proposed mutant enzymes were built and their Computational study predicted that Y176F/S241C double mutation of A novel functional double mutant ASNase with predicted improved enzymatic properties was designed and produced in
Identifiants
pubmed: 38022803
doi: 10.34172/apb.2023.085
pmc: PMC10676546
doi:
Types de publication
Journal Article
Langues
eng
Pagination
827-836Informations de copyright
©2023 The Authors.
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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