Best method to determine DNA G-quadruplex folding: The
Folding
G-quadruplex
Glycosidic conformation
HSQC
NMR
Journal
Methods (San Diego, Calif.)
ISSN: 1095-9130
Titre abrégé: Methods
Pays: United States
ID NLM: 9426302
Informations de publication
Date de publication:
27 Nov 2023
27 Nov 2023
Historique:
received:
22
09
2023
revised:
23
11
2023
accepted:
25
11
2023
pubmed:
30
11
2023
medline:
30
11
2023
entrez:
29
11
2023
Statut:
aheadofprint
Résumé
NMR spectroscopy is the major method for G-quadruplex structure determination under physiologically relevant solution conditions. Unlike duplex B-DNA, in which all nucleotides adopt an anti glycosidic conformation, the core tetrad-guanines in a G-quadruplex can adopt anti or syn glycosidic conformation depending on the folding structure. An experimental method that can clearly and unambiguously determine syn and anti tetrad-Gs in a G-quadruplex is highly desirable and necessary. In the present study, we exploit the advantages of the
Identifiants
pubmed: 38029869
pii: S1046-2023(23)00197-4
doi: 10.1016/j.ymeth.2023.11.013
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
35-41Informations de copyright
Copyright © 2023 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.