Effects of maternal type 1 diabetes and confounding factors on neonatal microbiomes.
HbA1c
Maternal reproductive tract microbiome
Microbiome
Microbiota transfer
Neonatal microbiome
Type 1 diabetes
Journal
Diabetologia
ISSN: 1432-0428
Titre abrégé: Diabetologia
Pays: Germany
ID NLM: 0006777
Informations de publication
Date de publication:
29 Nov 2023
29 Nov 2023
Historique:
received:
25
07
2023
accepted:
02
10
2023
medline:
30
11
2023
pubmed:
30
11
2023
entrez:
29
11
2023
Statut:
aheadofprint
Résumé
Body niche-specific microbiota in maternal-neonatal dyads from gravidae with type 1 diabetes have not been quantitatively and functionally examined. Similarly, the impact of pregnancy-specific factors, such as the presence of comorbidities known to occur more frequently among gravidae with type 1 diabetes, including Caesarean delivery, as well as antibiotic prophylaxis, level of glycaemic control during each trimester of pregnancy and insulin administration, has not been adequately considered. The aims of this study were to characterise the maternal and neonatal microbiomes, assess aspects of microbiota transfer from the maternal microbiomes to the neonatal microbiome and explore the impact of type 1 diabetes and confounding factors on the microbiomes. In this observational case-control study, we characterised microbiome community composition and function using 16S rRNA amplicon sequencing in a total of 514 vaginal, rectal and ear-skin swabs and stool samples derived from 92 maternal-neonatal dyads (including 50 gravidae with type 1 diabetes) and in-depth clinical metadata from throughout pregnancy and delivery. Type 1 diabetes-specific microbiota were identified among gravidae with type 1 diabetes and their neonates. Neonatal microbiome profiles of ear-skin swabs and stool samples were established, indicating the taxa more prevalent among neonates born to mothers with type 1 diabetes compared with neonates born to control mothers. Without taking into account the type 1 diabetes status of mothers, both delivery mode and intrapartum antibiotic prophylaxis were found to have an influence on neonatal microbiota composition (both p=0.001). In the logistic regression analysis involving all confounding variables, neonatal ear-skin microbiome variation was explained by maternal type 1 diabetes status (p=0.020) and small for gestational age birthweight (p=0.050). Moreover, in women with type 1 diabetes, a relationship was found between HbA These findings indicate that, in addition to maternal type 1 diabetes, glycaemic dysregulation before/in the first trimester of pregnancy, mode of delivery and intrapartum antibiotic prophylaxis may contribute to the inoculation and formation of the neonatal microbiomes. The BioProject (PRJNA961636) and associated SRA metadata are available at http://www.ncbi.nlm.nih.gov/bioproject/961636 . Processed data on probiotic supplementation and the PICRUSt analysis are available in the Mendeley Data Repository ( https://doi.org/10.17632/g68rwnnrfk.1 ).
Identifiants
pubmed: 38030736
doi: 10.1007/s00125-023-06047-7
pii: 10.1007/s00125-023-06047-7
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Poznan Supercomputing and Networking Center
ID : PSNC-534
Organisme : Polish Diabetes Association
ID : Professor Artur Czyżyk' Scientific Grant 2015
Organisme : Polish Diabetes Association
ID : Professor Artur Czyżyk' Scientific Grant 2017
Organisme : Uniwersytet Medyczny im. Karola Marcinkowskiego w Poznaniu
ID : 502-01-01110142-05618
Organisme : Uniwersytet Medyczny im. Karola Marcinkowskiego w Poznaniu
ID : 502-14-03301402-09911
Informations de copyright
© 2023. The Author(s).
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