A Novel Bifidobacterium/Klebsiella Ratio in Characterization Analysis of the Gut and Bile Microbiota of CCA Patients.
B/K ratio
Bile microbiome
Cholangiocarcinoma
Gut microbiome
Microbiomarkers
Journal
Microbial ecology
ISSN: 1432-184X
Titre abrégé: Microb Ecol
Pays: United States
ID NLM: 7500663
Informations de publication
Date de publication:
30 Nov 2023
30 Nov 2023
Historique:
received:
21
05
2023
accepted:
06
10
2023
medline:
1
12
2023
pubmed:
30
11
2023
entrez:
29
11
2023
Statut:
epublish
Résumé
Cholangiocarcinoma (CCA) is a serious health problem worldwide. The gut and bile microbiota have not been clearly characterized in patients with CCA, and better noninvasive diagnostic approaches for CCA need to be established. The aim of this study was to investigate the characteristics of the gut and bile microbiota in CCA patients. Forty-two CCA patients and 16 healthy normal controls (HNCs) were enrolled. DNA was extracted from fecal and bile samples and subjected to 16S rRNA gene analysis. We found that there were significant differences in the species diversity, structure, and composition of the microbial communities between the CCA group and the HNC grouAt the phylum level, compared with that in the HNC group, the relative abundance of Firmicutes and Actinobacteriota was significantly decreased in the CCA group, whereas Proteobacteria and Bacteroidota were significantly enriched. The Firmicutes/Bacteroidota (F/B) ratio significantly decreased in the CCA group compared to the HNC grouThe relative abundance of Klebsiella in the CCA group was significantly higher than that in the HNC group, while the relative abundance of Bifidobacterium was significantly decreased. The Bifidobacterium/Klebsiella (B/K) ratio was established as a novel biomarker and was found to be significantly decreased in the CCA group compared with the HNC grouOur findings provide evidence supporting the use of Klebsiella and Bifidobacterium as noninvasive intestinal microbiomarkers for improving the diagnosis of CCA.
Identifiants
pubmed: 38030815
doi: 10.1007/s00248-023-02318-3
pii: 10.1007/s00248-023-02318-3
pmc: PMC10687116
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
5Informations de copyright
© 2023. The Author(s).
Références
Clin Transl Gastroenterol. 2020 Dec 22;12(1):e00292
pubmed: 33464733
Annu Rev Pathol. 2021 Jan 24;16:433-463
pubmed: 33264573
Am J Physiol Gastrointest Liver Physiol. 2020 May 1;318(5):G889-G906
pubmed: 32146836
Liver Int. 2022 Jan;42(1):135-148
pubmed: 34459095
Pediatr Obes. 2021 Jun;16(6):e12750
pubmed: 33174684
PLoS One. 2023 Apr 18;18(4):e0283021
pubmed: 37071646
Ann Neurol. 2018 Jul;84(1):23-36
pubmed: 29733457
Gut. 2020 Apr;69(4):665-672
pubmed: 31243055
Front Cell Infect Microbiol. 2021 Nov 23;11:751795
pubmed: 34888258
World J Gastroenterol. 2015 Aug 7;21(29):8787-803
pubmed: 26269668
Gut. 2010 Dec;59(12):1635-42
pubmed: 20926643
Front Physiol. 2021 Nov 12;12:715536
pubmed: 34867436
PLoS One. 2016 Aug 23;11(8):e0161635
pubmed: 27551722
PLoS One. 2020 Nov 19;15(11):e0242553
pubmed: 33211762
Diagnostics (Basel). 2022 Nov 01;12(11):
pubmed: 36359498
World J Microbiol Biotechnol. 2021 Jul 2;37(8):128
pubmed: 34212246
Nat Rev Gastroenterol Hepatol. 2018 Jul;15(7):397-411
pubmed: 29748586
Nat Med. 2019 Mar;25(3):377-388
pubmed: 30842679
Gastroenterol Res Pract. 2021 Apr 08;2021:6647145
pubmed: 33927759
Ann Transl Med. 2021 Apr;9(8):644
pubmed: 33987342
Transl Cancer Res. 2021 Mar;10(3):1261-1272
pubmed: 35116453
Am J Cancer Res. 2021 Jun 15;11(6):3163-3175
pubmed: 34249452
Hepatol Int. 2022 Feb;16(1):99-111
pubmed: 35064546
Sci Rep. 2017 Aug 30;7(1):10054
pubmed: 28855583
Biochim Biophys Acta Mol Basis Dis. 2018 Apr;1864(4 Pt B):1468-1477
pubmed: 28782657
Microbiome. 2020 Aug 20;8(1):119
pubmed: 32819450
Scand J Surg. 2022 Jan-Mar;111(1):14574969211061953
pubmed: 34931568
Science. 2005 Mar 25;307(5717):1915-20
pubmed: 15790844
Crit Rev Food Sci Nutr. 2019;59(21):3456-3467
pubmed: 30010390
PLoS One. 2020 May 13;15(5):e0232930
pubmed: 32401801
Curr Gastroenterol Rep. 2011 Apr;13(2):182-7
pubmed: 21271364
Front Oncol. 2022 Jul 18;12:924696
pubmed: 35924173
Gastroenterology. 2014 May;146(6):1489-99
pubmed: 24560869
Science. 2018 Jan 5;359(6371):97-103
pubmed: 29097493
Gut Microbes. 2020 Nov 9;12(1):1857505
pubmed: 33382358
Biochim Biophys Acta Rev Cancer. 2021 Jan;1875(1):188494
pubmed: 33346129
Adv Cancer Res. 2022;156:39-73
pubmed: 35961707
Am J Gastroenterol. 2010 Oct;105(10):2218-27
pubmed: 20517305
Proc Natl Acad Sci U S A. 2007 Aug 21;104(34):13780-5
pubmed: 17699621
Front Med (Lausanne). 2022 Mar 17;9:836369
pubmed: 35372388
Br J Cancer. 2022 Mar;126(5):693-705
pubmed: 34663949
Nat Rev Dis Primers. 2021 Sep 9;7(1):65
pubmed: 34504109
Dis Markers. 2019 Apr 04;2019:7593560
pubmed: 31089397
Am J Transl Res. 2022 May 15;14(5):2962-2971
pubmed: 35702117
Front Microbiol. 2018 Nov 20;9:2777
pubmed: 30515141
PLoS One. 2017 Aug 10;12(8):e0182924
pubmed: 28796833
Int Immunopharmacol. 2020 Nov;88:106862
pubmed: 32771947