Single-cell analysis unveils activation of mast cells in colorectal cancer microenvironment.
Activation
Colorectal cancer
Heterogeneity
Mast cells
Prognosis
Single-cell analysis
Journal
Cell & bioscience
ISSN: 2045-3701
Titre abrégé: Cell Biosci
Pays: England
ID NLM: 101561195
Informations de publication
Date de publication:
29 Nov 2023
29 Nov 2023
Historique:
received:
07
06
2023
accepted:
09
10
2023
medline:
30
11
2023
pubmed:
30
11
2023
entrez:
30
11
2023
Statut:
epublish
Résumé
The role of mast cells (MCs) in colorectal cancer (CRC) remains unclear, and a comprehensive single-cell study on CRC MCs has not been conducted. This study used a multi-omics approach, integrating single-cell sequencing, spatial transcriptomics, and bulk tissue sequencing data to investigate the heterogeneity and impact of MCs in CRC. Five MC signature genes (TPSAB1, TPSB2, CPA3, HPGDS, and MS4A2) were identified, and their average expression was used as a marker of MCs. The MC density was found to be lower in CRC compared to normal tissue, but MCs in CRC demonstrated distinct activation features. Activated MCs were defined by high expression of receptors and MC mediators, while resting MCs had low expression. Most genes, including the five MC signature genes, were expressed at higher levels in activated MCs. The MC signature was linked to a better prognosis in both CRC and pan-cancer patient cohorts. Elevated KITLG expression was observed in fibroblasts and endothelial cells in CRC samples compared to normal tissue, and co-localization of MCs with these cell types was revealed by spatial transcriptome analysis. In conclusion, this study finds decreased MC density in CRC compared to normal tissue, but highlights a shift in MC phenotype from CMA1
Identifiants
pubmed: 38031173
doi: 10.1186/s13578-023-01144-x
pii: 10.1186/s13578-023-01144-x
pmc: PMC10687892
doi:
Types de publication
Journal Article
Langues
eng
Pagination
217Subventions
Organisme : This work was supported by the National Natural Science Foundation of China
ID : 82072655
Organisme : Scientific and technological innovation team of Shaanxi Innovation Capability Support Plan
ID : 2023-CX-TD-67
Organisme : Key R&D Plan of Shaanxi Province
ID : 2022SF-603
Informations de copyright
© 2023. The Author(s).
Références
Immunol Rev. 2009 Mar;228(1):149-69
pubmed: 19290926
Allergy. 2022 Jan;77(1):83-99
pubmed: 33955017
Nucleic Acids Res. 2019 Jul 2;47(W1):W556-W560
pubmed: 31114875
F1000Prime Rep. 2015 Jan 05;7:09
pubmed: 25705392
Int J Biol Sci. 2021 Sep 3;17(14):3837-3849
pubmed: 34671202
Nat Rev Immunol. 2013 May;13(5):362-75
pubmed: 23558889
Front Immunol. 2017 Apr 12;8:424
pubmed: 28446910
Mucosal Immunol. 2015 May;8(3):444-63
pubmed: 25669149
Nat Protoc. 2020 Apr;15(4):1484-1506
pubmed: 32103204
Nat Biotechnol. 2019 Jul;37(7):773-782
pubmed: 31061481
Blood. 1993 Feb 1;81(3):656-60
pubmed: 7678995
Int J Surg Pathol. 2013 Apr;21(2):111-20
pubmed: 22649166
J Allergy Clin Immunol. 2022 Jun;149(6):2062-2077
pubmed: 35304158
World J Gastroenterol. 2005 Feb 28;11(8):1210-4
pubmed: 15754407
JAMA Oncol. 2018 Mar 01;4(3):379-383
pubmed: 28983557
Biochim Biophys Acta Rev Cancer. 2023 Jan;1878(1):188845
pubmed: 36476563
Cell. 2021 Feb 4;184(3):792-809.e23
pubmed: 33545035
N Engl J Med. 2015 Jun 25;372(26):2509-20
pubmed: 26028255
FASEB J. 2014 Aug;28(8):3540-51
pubmed: 24760752
Int J Cancer. 2018 Nov 1;143(9):2271-2280
pubmed: 29873076
CA Cancer J Clin. 2022 Jan;72(1):7-33
pubmed: 35020204
Nat Methods. 2015 May;12(5):453-7
pubmed: 25822800
World J Gastroenterol. 2014 Jul 21;20(27):8910-20
pubmed: 25083063
Acta Oncol. 2016 Dec;55(12):1434-1442
pubmed: 27355473
Int J Cancer. 2015 Nov 1;137(9):2139-48
pubmed: 25921209
Nat Immunol. 2008 Nov;9(11):1215-23
pubmed: 18936782
Eur J Immunol. 2010 Jul;40(7):1843-51
pubmed: 20583030
J Innate Immun. 2021;13(3):131-147
pubmed: 33582673
Nat Immunol. 2023 Jun;24(6):915-924
pubmed: 37081147
Cell Mol Immunol. 2020 Aug;17(8):807-821
pubmed: 32612154
J Allergy Clin Immunol. 2018 Jan;141(1):92-93
pubmed: 28624609
Annu Rev Immunol. 2021 Apr 26;39:583-609
pubmed: 33637019
Cells. 2023 Jan 31;12(3):
pubmed: 36766801
Expert Rev Clin Immunol. 2019 Jun;15(6):639-656
pubmed: 30884251
Front Immunol. 2020 Jul 03;11:1389
pubmed: 32719677
Eur Rev Med Pharmacol Sci. 2021 Nov;25(22):6862-6873
pubmed: 34859861
Oncogene. 2023 Jan;42(3):209-223
pubmed: 36402931
Cell. 2020 Apr 16;181(2):442-459.e29
pubmed: 32302573
Blood. 2009 Dec 3;114(24):4979-88
pubmed: 19805617
BMC Bioinformatics. 2013 Jan 16;14:7
pubmed: 23323831
Nat Biotechnol. 2014 Apr;32(4):381-386
pubmed: 24658644
Oncoimmunology. 2017 Aug 8;6(11):e1360457
pubmed: 29147625
Blood. 2008 Aug 15;112(4):1269-79
pubmed: 18524989
Nat Commun. 2019 Jun 21;10(1):2735
pubmed: 31227713
Nature. 2018 Dec;564(7735):268-272
pubmed: 30479382
Nat Rev Cancer. 2020 Nov;20(11):662-680
pubmed: 32753728
Cells. 2021 May 21;10(6):
pubmed: 34063789
Leukemia. 2013 Feb;27(2):278-85
pubmed: 22907049
Proc Natl Acad Sci U S A. 1986 Jun;83(12):4464-8
pubmed: 3520574
Acta Pathol Microbiol Scand. 1966;66(3):289-302
pubmed: 4162017
Int J Mol Sci. 2019 Sep 06;20(18):
pubmed: 31500217
J Gastroenterol Hepatol. 2009 Jul;24(7):1265-75
pubmed: 17645466
BMC Cancer. 2015 Nov 04;15:840
pubmed: 26530140