Tetraploid embryo aggregation produces high-quality blastocysts with an increased trophectoderm in pigs.
blastomere aggregation
parthenogenetic activation
pig
tetraploid
trophectoderm
Journal
Frontiers in cell and developmental biology
ISSN: 2296-634X
Titre abrégé: Front Cell Dev Biol
Pays: Switzerland
ID NLM: 101630250
Informations de publication
Date de publication:
2023
2023
Historique:
received:
13
06
2023
accepted:
01
11
2023
medline:
30
11
2023
pubmed:
30
11
2023
entrez:
30
11
2023
Statut:
epublish
Résumé
Tetraploid complementation is an ideal method for demonstrating the differentiation potential of pluripotent stem cells. In this study, we selected the most efficient tetraploid production method for porcine embryos and investigated whether tetraploid blastomere aggregation could enhance the quality of tetraploid embryos. Three methods were investigated to produce tetraploid embryos: First, tetraploid embryos were produced using electro-fusion of two-cell stage parthenogenetic blastomere (FUTP). Second, somatic cell was injected into the mature oocyte and fused to produce tetraploid embryos. Third, oocytes were matured with Cytochalasin B (CB) for the late 22 h of
Identifiants
pubmed: 38033873
doi: 10.3389/fcell.2023.1239448
pii: 1239448
pmc: PMC10687364
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1239448Informations de copyright
Copyright © 2023 Lee, Cai, Kim, Choi, Oh, Jawad, Lee and Hyun.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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