Understanding wax ester synthesis in Euglena gracilis: Insights into mitochondrial anaerobic respiration.

Anaerobic respiration Euglena gracilis Nicotinamide nucleotide transhydrogenase Rhodoquinone Wax ester fermentation

Journal

Protist

ISSN: 1618-0941

Titre abrégé: Protist

Pays: Germany

ID NLM: 9806488

Informations de publication

Date de publication:
Dec 2023

Historique:

received: 04 09 2023

revised: 31 10 2023

accepted: 20 11 2023

pubmed: 3 12 2023

medline: 3 12 2023

entrez: 2 12 2023

Statut: ppublish

Résumé

Euglena gracilis, photosynthetic protist, has a unique ability to generate wax esters in the absence of oxygen, employing a distinctive fatty acid synthesis mechanism. Through comprehensive inhibitor assays and gene-silencing techniques, our research clearly emphasized the indispensable role of the mitochondrial anaerobic respiratory chain in this biosynthesis. We identified acyl-CoA dehydrogenase, electron transfer flavoprotein (ETF), and rhodoquinone (RQ) as central molecular components in the pathway. These findings strongly indicated a potential reversal of beta-oxidation occurring within mitochondria for fatty acid production in anaerobic conditions. Furthermore, our analysis revealed the pivotal function of nicotinamide nucleotide transhydrogenase (NNT) in efficiently managing the NADPH/NAD

Identifiants

DOI: 10.1016/j.protis.2023.125996 PMID: 38041972

pubmed: 38041972

pii: S1434-4610(23)00058-5

doi: 10.1016/j.protis.2023.125996

pii:

doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

125996

Informations de copyright

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.