Mechanistic study on ursolic acid inhibiting the growth of colorectal cancer cells through the downregulation of TGF-β3 by miR-140-5p.
TGF-β3
colorectal cancer
miR-140-5p
ursolic acid
Journal
Journal of biochemical and molecular toxicology
ISSN: 1099-0461
Titre abrégé: J Biochem Mol Toxicol
Pays: United States
ID NLM: 9717231
Informations de publication
Date de publication:
03 Dec 2023
03 Dec 2023
Historique:
revised:
05
09
2023
received:
06
12
2022
accepted:
03
11
2023
medline:
4
12
2023
pubmed:
4
12
2023
entrez:
3
12
2023
Statut:
aheadofprint
Résumé
Colorectal cancer (CRC) is a common digestive tract tumor with a high incidence and a poor prognosis. Traditional chemotherapy drugs are usually accompanied by unpleasant side effects, highlighting the importance of exploring new adjunctive drugs. In this study, we aimed to explore the role of ursolic acid (UA) in CRC cells. Specifically, HT-29 cells were treated with UA at different concentrations (10, 20, 30, and 40 μM), and the expression of miR-140-5p, tumor growth factor-β3 (TGF-β3), β-catenin, and cyclin D1 was determined by real-time quantitative PCR. The cell cycle and apoptosis were checked by flow cytometry, and cell proliferation was detected by Cell Counting Kit-8 assay. The HT-29 cell model was established through overexpression (miR-140-5p mimics) and interference (miR-140-5p inhibitor) of miR-140-5p. Western blot was used to detect the protein expression of TGF-β3. We found that UA could inhibit the proliferation of HT-29 cells, block cells in the G
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e23581Subventions
Organisme : Scientific Research Project of Putuo Hospital, Shanghai University of Traditional Chinese Medicine
ID : 2020371B
Organisme : One Hundred Talents Project of Putuo Hospital, Shanghai University of Traditional Chinese Medicine
ID : 2022-RCQH-03
Organisme : Science, Technology Innovation Project of Putuo District Health System
ID : ptkwws202307
Informations de copyright
© 2023 Wiley Periodicals LLC.
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