Dicer-dependent heterochromatic small RNAs in the model diatom species Phaeodactylum tricornutum.
Dicer
Phaeodactylum tricornutum
RNA interference
epigenetic
nitrate
small RNAs
transposon
Journal
The New phytologist
ISSN: 1469-8137
Titre abrégé: New Phytol
Pays: England
ID NLM: 9882884
Informations de publication
Date de publication:
03 Dec 2023
03 Dec 2023
Historique:
received:
06
09
2023
accepted:
17
10
2023
medline:
4
12
2023
pubmed:
4
12
2023
entrez:
4
12
2023
Statut:
aheadofprint
Résumé
Diatoms are eukaryotic microalgae responsible for nearly half of the marine productivity. RNA interference (RNAi) is a mechanism of regulation of gene expression mediated by small RNAs (sRNAs) processed by the endoribonuclease Dicer (DCR). To date, the mechanism and physiological role of RNAi in diatoms are unknown. We mined diatom genomes and transcriptomes for key RNAi effectors and retraced their phylogenetic history. We generated DCR knockout lines in the model diatom species Phaeodactylum tricornutum and analyzed their mRNA and sRNA populations, repression-associated histone marks, and acclimatory response to nitrogen starvation. Diatoms presented a diversification of key RNAi effectors whose distribution across species suggests the presence of distinct RNAi pathways. P. tricornutum DCR was found to process 26-31-nt-long double-stranded sRNAs originating mostly from transposons covered by repression-associated epigenetic marks. In parallel, P. tricornutum DCR was necessary for the maintenance of the repression-associated histone marks H3K9me2/3 and H3K27me3. Finally, PtDCR-KO lines presented a compromised recovery post nitrogen starvation suggesting a role for P. tricornutum DCR in the acclimation to nutrient stress. Our study characterized the molecular function of the single DCR homolog of P. tricornutum suggesting an association between RNAi and heterochromatin maintenance in this model diatom species.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Agence Nationale de la Recherche
ID : Initiative d'Excellence - DYNAMO/ANR-11-LABX-0
Organisme : European Molecular Biology Organization
ID : Short-Term Fellowships
Organisme : European Regional Development Fund
ID : CMBR/MIS 5002670
Organisme : Fondation Bettencourt-Schueller
ID : Coups d'élan pour la recherche francaise-2018
Organisme : General Secretariat for Research and Technology
ID : RADIO/483
Organisme : H2020 Marie Skłodowska-Curie Actions
ID : 734708/GHANA/H2020-MSCA-RISE-2016
Organisme : Hellenic Foundation for Research and Innovation
ID : RADIO/483
Organisme : Japan Society for the Promotion of Science
ID : BRIDGE fellowship BR220203
Informations de copyright
© 2023 The Authors. New Phytologist © 2023 New Phytologist Foundation.
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