Repeat mediated excision of gene drive elements for restoring wild-type populations.
CRISPR
Cas9
DNA Repair
Direct Repeats
Drosophila
Gene drive
HR
Homologous Recombination
SSA
Single Strand Annealing
autonomous
double-strand break
homing
Journal
bioRxiv : the preprint server for biology
Titre abrégé: bioRxiv
Pays: United States
ID NLM: 101680187
Informations de publication
Date de publication:
23 Nov 2023
23 Nov 2023
Historique:
pubmed:
4
12
2023
medline:
4
12
2023
entrez:
4
12
2023
Statut:
epublish
Résumé
We demonstrate here that single strand annealing (SSA) repair can be co-opted for the precise autocatalytic excision of a drive element. Although SSA is not the predominant form of DNA repair in eukaryotic organisms, we increased the likelihood of its use by engineering direct repeats at sites flanking the drive allele, and then introducing a double-strand DNA break (DSB) at a second endonuclease target site encoded within the drive allele. We have termed this technology Repeat Mediated Excision of a Drive Element (ReMEDE). Incorporation of ReMEDE into the previously described mutagenic chain reaction (MCR) gene drive, targeting the
Identifiants
pubmed: 38045402
doi: 10.1101/2023.11.23.568397
pmc: PMC10690251
pii:
doi:
Types de publication
Preprint
Langues
eng
Subventions
Organisme : NIAID NIH HHS
ID : R01 AI119081
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI148787
Pays : United States
Déclaration de conflit d'intérêts
Competing Interests KMM and ZNA are inventors on US provisional patent application PCT/US2021/041951, submitted by Texas A&M University, which covers vector constructs that are pre-programmed to self-eliminate or self-remove at a predetermined time, and methods of making the same. PRC, JZ, RDM, MLNM declare no competing interests.
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