Fast HPLC-based affinity method to determine capsid titer and full/empty ratio of adeno-associated viral vectors.

AAV adeno-associated affinity chromatography capsid titer fluorescence gene therapy high-throughput process development

Journal

Molecular therapy. Methods & clinical development
ISSN: 2329-0501
Titre abrégé: Mol Ther Methods Clin Dev
Pays: United States
ID NLM: 101624857

Informations de publication

Date de publication:
14 Dec 2023
Historique:
received: 02 06 2023
accepted: 31 10 2023
medline: 4 12 2023
pubmed: 4 12 2023
entrez: 4 12 2023
Statut: epublish

Résumé

Recombinant adeno-associated viruses (rAAVs) are promising gene delivery vectors in the emerging field of

Identifiants

pubmed: 38046198
doi: 10.1016/j.omtm.2023.101148
pii: S2329-0501(23)00187-0
pmc: PMC10690635
doi:

Types de publication

Journal Article

Langues

eng

Pagination

101148

Informations de copyright

© 2023 The Authors.

Déclaration de conflit d'intérêts

The authors declare no competing interests.

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Auteurs

Jakob Heckel (J)

Pharma Technical Development Analytics, Roche Diagnostics GmbH, 82377 Penzberg, Germany.

Andres Martinez (A)

Gene Therapy Technical Development, Roche Diagnostics GmbH, 82377 Penzberg, Germany.

Carsten Elger (C)

Gene Therapy Technical Development, Roche Diagnostics GmbH, 82377 Penzberg, Germany.

Markus Haindl (M)

Gene Therapy Technical Development, Roche Diagnostics GmbH, 82377 Penzberg, Germany.

Michael Leiss (M)

Pharma Technical Development Analytics, Roche Diagnostics GmbH, 82377 Penzberg, Germany.

Raphael Ruppert (R)

Gene Therapy Technical Development, Roche Diagnostics GmbH, 82377 Penzberg, Germany.

Chris Williams (C)

Gene Therapy Technical Development, Roche Diagnostics GmbH, 82377 Penzberg, Germany.

Jürgen Hubbuch (J)

Institute of Process Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology, 76131 Karlsruhe, Germany.

Tobias Graf (T)

Pharma Technical Development Analytics, Roche Diagnostics GmbH, 82377 Penzberg, Germany.

Classifications MeSH