ANCA: artificial nucleic acid circuit with argonaute protein for one-step isothermal detection of antibiotic-resistant bacteria.
Humans
Anti-Bacterial Agents
/ pharmacology
Bacterial Proteins
/ genetics
beta-Lactamases
/ genetics
Argonaute Proteins
/ genetics
Antibodies, Antineutrophil Cytoplasmic
/ metabolism
Nucleic Acids
/ metabolism
Bacteria
/ genetics
DNA
/ metabolism
Klebsiella pneumoniae
/ genetics
Microbial Sensitivity Tests
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
05 Dec 2023
05 Dec 2023
Historique:
received:
15
06
2023
accepted:
23
11
2023
medline:
7
12
2023
pubmed:
6
12
2023
entrez:
5
12
2023
Statut:
epublish
Résumé
Endonucleases have recently widely used in molecular diagnostics. Here, we report a strategy to exploit the properties of Argonaute (Ago) proteins for molecular diagnostics by introducing an artificial nucleic acid circuit with Ago protein (ANCA) method. The ANCA is designed to perform a continuous autocatalytic reaction through cross-catalytic cleavage of the Ago protein, enabling one-step, amplification-free, and isothermal DNA detection. Using the ANCA method, carbapenemase-producing Klebsiella pneumoniae (CPKP) are successfully detected without DNA extraction and amplification steps. In addition, we demonstrate the detection of carbapenem-resistant bacteria in human urine and blood samples using the method. We also demonstrate the direct identification of CPKP swabbed from surfaces using the ANCA method in conjunction with a three-dimensional nanopillar structure. Finally, the ANCA method is applied to detect CPKP in rectal swab specimens from infected patients, achieving sensitivity and specificity of 100% and 100%, respectively. The developed method can contribute to simple, rapid and accurate diagnosis of CPKP, which can help prevent nosocomial infections.
Identifiants
pubmed: 38052830
doi: 10.1038/s41467-023-43899-4
pii: 10.1038/s41467-023-43899-4
pmc: PMC10697997
doi:
Substances chimiques
Anti-Bacterial Agents
0
Bacterial Proteins
0
beta-Lactamases
EC 3.5.2.6
Argonaute Proteins
0
Antibodies, Antineutrophil Cytoplasmic
0
Nucleic Acids
0
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
8033Subventions
Organisme : National Research Foundation of Korea (NRF)
ID : NRF-2021M3H4A1A02051048
Organisme : National Research Foundation of Korea (NRF)
ID : NRF-2023R1A2C2005185
Organisme : National Research Foundation of Korea (NRF)
ID : NRF-2021M3E5E3080844
Organisme : National Research Foundation of Korea (NRF)
ID : NRF-2022R1C1C1008815
Organisme : National Research Council of Science and Technology (National Research Council of Science & Technology)
ID : CPS22021-100
Organisme : MOE | Korea Environmental Industry and Technology Institute (KEITI)
ID : 2021003370003
Organisme : Ministry of Trade, Industry and Energy, Korea | Korea Evaluation Institute of Industrial Technology (KEIT)
ID : RS-2022-00154853
Organisme : Korea Research Institute of Bioscience and Biotechnology (KRIBB)
ID : KGM5472322
Informations de copyright
© 2023. The Author(s).
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