Abcc6 deficiency prevents rhabdomyolysis-induced acute kidney injury.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
06 Dec 2023
Historique:
received: 06 03 2023
accepted: 20 11 2023
medline: 11 12 2023
pubmed: 7 12 2023
entrez: 6 12 2023
Statut: epublish

Résumé

Rhabdomyolysis is a risk factor for acute kidney injury, transition towards chronic kidney disease, and death. The role of calcium phosphate deposits in the mechanisms of rhabdomyolysis-induced acute kidney injury (RAKI) is still unclear. Better insight of the role calcium in RAKI could lead to new therapeutic avenues. Here, we show in a mice model of RAKI that calcium phosphate deposits were frequent in the kidney (hydroxyapatite) and partly correlated with the severity of the kidney injury. However, the intensity of deposits was highly heterogeneous between mice. Treatment with sodium chloride, sodium bicarbonate or inorganic pyrophosphate (PPi; an inhibitor of the calcium phosphate crystallization), or combinations thereof, did not improve kidney outcomes and hydroxyapatite deposition during RAKI. Unexpectedly, Abcc6 knockout mice (ko), characterized by PPi deficiency, developed less severe RAKI despite similar rhabdomyolysis severity, and had similar hydroxyapatite deposition suggesting alternative mechanisms. This improved kidney outcome at day 2 translated to a trend in improved glomerular filtration rate at month 2 in Abcc6

Identifiants

pubmed: 38057332
doi: 10.1038/s41598-023-47894-z
pii: 10.1038/s41598-023-47894-z
pmc: PMC10700332
doi:

Substances chimiques

Sodium Bicarbonate 8MDF5V39QO
Sodium Chloride 451W47IQ8X
Calcium Phosphates 0
Hydroxyapatites 0
Abcc6 protein, mouse 0
Multidrug Resistance-Associated Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

21513

Subventions

Organisme : Université Toulouse III - Paul Sabatier
ID : Ecole doctorale BSB
Organisme : ERA PerMed-JTC 2018
ID : ANR-18-PERM-0003
Organisme : ERA PerMed-JTC 2018
ID : ANR-18-PERM-0003
Organisme : Fondation pour la Recherche Médicale
ID : DEQ20170336759

Informations de copyright

© 2023. The Author(s).

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Auteurs

Audrey Casemayou (A)

Department of Nephrology and Organ Transplantation, Reference Centre for Rare Kidney Diseases (SORARE), French Intensive Care Renal Network (FIRN), University Hospital of Toulouse, 1, Avenue du Pr. Jean Poulhes, 31059, Toulouse Cedex, France.
National Institute of Health and Medical Research (INSERM), Institute of Metabolic and Cardiovascular Diseases (I2MC), UMR 1297, Rangueil Hospital, 31000, Toulouse, France.
University Toulouse-3, 31000, Toulouse, France.

Julie Belliere (J)

Department of Nephrology and Organ Transplantation, Reference Centre for Rare Kidney Diseases (SORARE), French Intensive Care Renal Network (FIRN), University Hospital of Toulouse, 1, Avenue du Pr. Jean Poulhes, 31059, Toulouse Cedex, France.
National Institute of Health and Medical Research (INSERM), Institute of Metabolic and Cardiovascular Diseases (I2MC), UMR 1297, Rangueil Hospital, 31000, Toulouse, France.
University Toulouse-3, 31000, Toulouse, France.

Emmanuel Letavernier (E)

National Institute of Health and Medical Research, (INSERM) UMR S 1155, Tenon Hospital, 75020, Paris, France.
Sorbonne University, 75020, Paris, France.
Department of Physiology, Tenon Hospital, Assistance Publique - Hôpitaux de Paris, 75020, Paris, France.

Eloïse Colliou (E)

Department of Nephrology and Organ Transplantation, Reference Centre for Rare Kidney Diseases (SORARE), French Intensive Care Renal Network (FIRN), University Hospital of Toulouse, 1, Avenue du Pr. Jean Poulhes, 31059, Toulouse Cedex, France.
National Institute of Health and Medical Research (INSERM), Institute of Metabolic and Cardiovascular Diseases (I2MC), UMR 1297, Rangueil Hospital, 31000, Toulouse, France.
University Toulouse-3, 31000, Toulouse, France.

Hélène El Hachem (H)

Department of Nephrology and Organ Transplantation, Reference Centre for Rare Kidney Diseases (SORARE), French Intensive Care Renal Network (FIRN), University Hospital of Toulouse, 1, Avenue du Pr. Jean Poulhes, 31059, Toulouse Cedex, France.
National Institute of Health and Medical Research (INSERM), Institute of Metabolic and Cardiovascular Diseases (I2MC), UMR 1297, Rangueil Hospital, 31000, Toulouse, France.

Jeremy Zarowski (J)

Department of Physiology, Tenon Hospital, Assistance Publique - Hôpitaux de Paris, 75020, Paris, France.

Dominique Bazin (D)

Laboratory of Chemistry and Physics, CNRS UMR 8000, University Paris XI, 91405, Orsay, France.

Clément Kounde (C)

Department of Nephrology and Organ Transplantation, Reference Centre for Rare Kidney Diseases (SORARE), French Intensive Care Renal Network (FIRN), University Hospital of Toulouse, 1, Avenue du Pr. Jean Poulhes, 31059, Toulouse Cedex, France.
National Institute of Health and Medical Research (INSERM), Institute of Metabolic and Cardiovascular Diseases (I2MC), UMR 1297, Rangueil Hospital, 31000, Toulouse, France.

Alexis Piedrafita (A)

Department of Nephrology and Organ Transplantation, Reference Centre for Rare Kidney Diseases (SORARE), French Intensive Care Renal Network (FIRN), University Hospital of Toulouse, 1, Avenue du Pr. Jean Poulhes, 31059, Toulouse Cedex, France.
National Institute of Health and Medical Research (INSERM), Institute of Metabolic and Cardiovascular Diseases (I2MC), UMR 1297, Rangueil Hospital, 31000, Toulouse, France.
University Toulouse-3, 31000, Toulouse, France.

Guylène Feuillet (G)

National Institute of Health and Medical Research (INSERM), Institute of Metabolic and Cardiovascular Diseases (I2MC), UMR 1297, Rangueil Hospital, 31000, Toulouse, France.

Joost P Schanstra (JP)

National Institute of Health and Medical Research (INSERM), Institute of Metabolic and Cardiovascular Diseases (I2MC), UMR 1297, Rangueil Hospital, 31000, Toulouse, France.
Department of Physiology, Tenon Hospital, Assistance Publique - Hôpitaux de Paris, 75020, Paris, France.

Stanislas Faguer (S)

Department of Nephrology and Organ Transplantation, Reference Centre for Rare Kidney Diseases (SORARE), French Intensive Care Renal Network (FIRN), University Hospital of Toulouse, 1, Avenue du Pr. Jean Poulhes, 31059, Toulouse Cedex, France. stanislas.faguer@inserm.fr.
National Institute of Health and Medical Research (INSERM), Institute of Metabolic and Cardiovascular Diseases (I2MC), UMR 1297, Rangueil Hospital, 31000, Toulouse, France. stanislas.faguer@inserm.fr.
Department of Physiology, Tenon Hospital, Assistance Publique - Hôpitaux de Paris, 75020, Paris, France. stanislas.faguer@inserm.fr.

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