Diagnosis of pleural tuberculosis by multi-targeted loop-mediated isothermal amplification assay based on SYBR Green I reaction: comparison with GeneXpert® MTB/RIF assay.

Diagnosis GeneXpert Mtb SYBR Green I SYBR Green I+dUTP-UNG assay dry methyl green multi-targeted LAMP pleural TB

Journal

Expert review of respiratory medicine
ISSN: 1747-6356
Titre abrégé: Expert Rev Respir Med
Pays: England
ID NLM: 101278196

Informations de publication

Date de publication:
07 Dec 2023
Historique:
pubmed: 7 12 2023
medline: 7 12 2023
entrez: 7 12 2023
Statut: aheadofprint

Résumé

Diagnosis of pleural tuberculosis (TB) is tedious owing to its close resemblance with malignant pleural effusion and sparse bacterial load in clinical specimens. There is an immediate need to design a rapid and dependable diagnostic test to prevent unnecessary morbidity/mortality. A multi-targeted loop-mediated isothermal amplification (MT-LAMP) was deliberated using mpt64 and IS6110 to diagnose pleural TB within pleural fluids/biopsies. MT-LAMP products were analyzed by gel-based and visual detection methods, viz. SYBR Green I, SYBR Green I+deoxyuridine triphosphate uracil-N-glycosylase (dUTP-UNG), and dry methyl green reactions. In a pilot study, while assessing pleural TB/non-TB control subjects ( Our SYBR Green I+dUTP-UNG MT-LAMP is a simple and reliable method to diagnose pleural TB, which may translate into a point-of-care test.

Sections du résumé

BACKGROUND UNASSIGNED
Diagnosis of pleural tuberculosis (TB) is tedious owing to its close resemblance with malignant pleural effusion and sparse bacterial load in clinical specimens. There is an immediate need to design a rapid and dependable diagnostic test to prevent unnecessary morbidity/mortality.
RESEARCH DESIGN AND METHODS UNASSIGNED
A multi-targeted loop-mediated isothermal amplification (MT-LAMP) was deliberated using mpt64 and IS6110 to diagnose pleural TB within pleural fluids/biopsies. MT-LAMP products were analyzed by gel-based and visual detection methods, viz. SYBR Green I, SYBR Green I+deoxyuridine triphosphate uracil-N-glycosylase (dUTP-UNG), and dry methyl green reactions.
RESULTS UNASSIGNED
In a pilot study, while assessing pleural TB/non-TB control subjects (
CONCLUSIONS UNASSIGNED
Our SYBR Green I+dUTP-UNG MT-LAMP is a simple and reliable method to diagnose pleural TB, which may translate into a point-of-care test.

Identifiants

DOI: 10.1080/17476348.2023.2292738 PMID: 38058175
pubmed: 38058175
doi: 10.1080/17476348.2023.2292738
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1-11

Auteurs

Aishwarya Soni (A)

Centre for Biotechnology, Maharshi Dayanand University (MDU), Rohtak, India.
Department of Biotechnology, Deenbandhu Chhotu Ram University of Science and Technology (DCRUST), Murthal, Sonipat, India.

Bhawna Dahiya (B)

Centre for Biotechnology, Maharshi Dayanand University (MDU), Rohtak, India.
Microbiology Department, Faculty of Allied Health Sciences, Shree Guru Gobind Singh Tricentenary (SGT) University, Gurugram, India.

Abhishek Sheoran (A)

Department of Statistics, Ramanujan College, University of Delhi, New Delhi, India.

Vipul Kumar (V)

Department of TB & Respiratory Medicine, University of Health Sciences (UHS), Rohtak, India.

Astha Guliani (A)

Department of TB & Respiratory Medicine, University of Health Sciences (UHS), Rohtak, India.

Nitin Kumar (N)

Centre for Medical Biotechnology, MDU, Rohtak, India.

Vikas Hooda (V)

Centre for Biotechnology, Maharshi Dayanand University (MDU), Rohtak, India.

Aparna Yadav (A)

Microbiology Department, UHS, Rohtak, India.

Kiran Nehra (K)

Department of Biotechnology, Deenbandhu Chhotu Ram University of Science and Technology (DCRUST), Murthal, Sonipat, India.

Promod K Mehta (PK)

Centre for Biotechnology, Maharshi Dayanand University (MDU), Rohtak, India.
Microbiology Department, Faculty of Allied Health Sciences, Shree Guru Gobind Singh Tricentenary (SGT) University, Gurugram, India.

Classifications MeSH