Construction of Infectious Clones for Human Enteroviruses.

DNA-launch plasmids Enterovirus Fluorescence reporter gene Infectious clone Reverse genetics

Journal

Methods in molecular biology (Clifton, N.J.)

ISSN: 1940-6029

Titre abrégé: Methods Mol Biol

Pays: United States

ID NLM: 9214969

Informations de publication

Date de publication:
2024

Historique:

medline: 8 12 2023

pubmed: 8 12 2023

entrez: 8 12 2023

Statut: ppublish

Résumé

The infectious clone has been constructed for years via various mechanisms using reverse genetics of viral RNA into cDNA. The mechanism of construction has evolved to DNA-launch plasmids which simplify infectious clone manipulation and expression in mammalian cells. Infectious clones have enormously allowed manipulation of the enterovirus genome to discover antivirals, viral replication mechanisms, and functions of essential viral proteins. Here we will be discussing methods for the production of DNA-launch human enterovirus infectious clones and viral genome engineered with a fluorescent reporter gene.

Identifiants

DOI: 10.1007/978-1-0716-3533-9_10 PMID: 38064032

pubmed: 38064032

doi: 10.1007/978-1-0716-3533-9_10

doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

155-174

Informations de copyright

Références

Romero JR (2017) 164—Human enteroviruses. In: Cohen J, Powderly WG, Opal SM (eds) Infectious diseases, 4th edn. Elsevier, p 1406–1416.e1401. https://doi.org/10.1016/B978-0-7020-6285-8.00164-7

doi: 10.1016/B978-0-7020-6285-8.00164-7

Nikonov OS, Chernykh ES, Garber MB, Nikonova EY (2017) Enteroviruses: classification, diseases they cause, and approaches to development of antiviral drugs. Biochemistry (Mosc) 82(13):1615–1631. https://doi.org/10.1134/S0006297917130041

doi: 10.1134/S0006297917130041 pubmed: 29523062

Solomon T, Lewthwaite P, Perera D, Cardosa MJ, McMinn P, Ooi MH (2010) Virology, epidemiology, pathogenesis, and control of enterovirus 71. Lancet Infect Dis 10(11):778–790. https://doi.org/10.1016/S1473-3099(10)70194-8

doi: 10.1016/S1473-3099(10)70194-8 pubmed: 20961813

Yuan J, Shen L, Wu J, Zou X, Gu J, Chen J, Mao L (2018) Enterovirus A71 proteins: structure and function. Front Microbiol 9

van der Linden L, Wolthers KC, van Kuppeveld FJ (2015) Replication and inhibitors of enteroviruses and parechoviruses. Viruses 7(8):4529–4562. https://doi.org/10.3390/v7082832

doi: 10.3390/v7082832 pubmed: 26266417 pmcid: 4576193

Gunaseelan S, Wong KZ, Min N, Sun J, Ismail N, Tan YJ, Lee RCH, Chu JJH (2019) Prunin suppresses viral IRES activity and is a potential candidate for treating enterovirus A71 infection. Sci Transl Med 11(516). https://doi.org/10.1126/scitranslmed.aar5759

Tsai YH, Huang SW, Hsieh WS, Cheng CK, Chang CF, Wang YF, Wang JR (2019) Enterovirus A71 containing codon-deoptimized VP1 and high-fidelity polymerase as next-generation vaccine candidate. J Virol 93(13). https://doi.org/10.1128/jvi.02308-18

Yogarajah T, Ong KC, Perera D, Wong KT (2018) RSAD2 and AIM2 modulate coxsackievirus A16 and enterovirus A71 replication in neuronal cells in different ways that may be associated with their 5′ nontranslated regions. J Virol 92(6). https://doi.org/10.1128/jvi.01914-17

Utt A, Rausalu K, Jakobson M, Männik A, Alphey L, Fragkoudis R, Merits A (2019) Design and use of chikungunya virus replication templates utilizing mammalian and mosquito RNA polymerase I-mediated transcription. J Virol 93(18):e00794–e00719. https://doi.org/10.1128/JVI.00794-19

doi: 10.1128/JVI.00794-19 pubmed: 31217251 pmcid: 6714806

Qin Z, Chen Y, Yu J, He X, Liu X, Zhu L, Wu Q, Wan C, Zhang B, Zhao W (2021) Rapid construction of an infectious clone of the Zika virus, strain ZKC2. Front Microbiol 12

Chin W-X, Lee RCH, Kaur P, Lew TS, Yogarajah T, Kong HY, Teo Z-Y, Salim CK, Zhang R-R, Li X-F, Alonso S, Qin C-F, Chu JJH (2021) A single-dose live attenuated chimeric vaccine candidate against Zika virus. NPJ Vaccines 6(1):20–20. https://doi.org/10.1038/s41541-021-00282-y

doi: 10.1038/s41541-021-00282-y pubmed: 33514743 pmcid: 7846741

Zimina A, Viktorova EG, Moghimi S, Nchoutmboube J, Belov GA (2021) Interaction of poliovirus capsid proteins with the cellular autophagy pathway. Viruses 13(8). https://doi.org/10.3390/v13081587

van der Schaar HM, Melia CE, van Bruggen JA, Strating JR, van Geenen ME, Koster AJ, Bárcena M, van Kuppeveld FJ (2016) Illuminating the sites of enterovirus replication in living cells by using a split-GFP-tagged viral protein. mSphere 1(4). https://doi.org/10.1128/mSphere.00104-16

Meister SW, Hendrikse NM, Löfblom J (2019) Directed evolution of the 3C protease from coxsackievirus using a novel fluorescence-assisted intracellular method. Biol Chem 400(3):405–415. https://doi.org/10.1515/hsz-2018-0362

doi: 10.1515/hsz-2018-0362 pubmed: 30521472

Schürer H, Lang K, Schuster J, Mörl M (2002) A universal method to produce in vitro transcripts with homogeneous 3′ ends. Nucleic Acids Res 30(12):e56. https://doi.org/10.1093/nar/gnf055

doi: 10.1093/nar/gnf055 pubmed: 12060694

Walker SC, Avis JM, Conn GL (2003) General plasmids for producing RNA in vitro transcripts with homogeneous ends. Nucleic Acids Res 31(15):e82. https://doi.org/10.1093/nar/gng082

doi: 10.1093/nar/gng082 pubmed: 12888534 pmcid: 169970

Al-Allaf FA, Tolmachov OE, Zambetti LP, Tchetchelnitski V, Mehmet H (2013) Remarkable stability of an instability-prone lentiviral vector plasmid in Escherichia coli Stbl3. 3 Biotech 3(1):61–70. https://doi.org/10.1007/s13205-012-0070-8

doi: 10.1007/s13205-012-0070-8 pubmed: 28324350