Nutrient deprivation alters the rate of COPII subunit recruitment at ER subdomains to tune secretory protein transport.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
08 Dec 2023
08 Dec 2023
Historique:
received:
03
03
2023
accepted:
27
11
2023
medline:
9
12
2023
pubmed:
9
12
2023
entrez:
8
12
2023
Statut:
epublish
Résumé
Co-assembly of the multilayered coat protein complex II (COPII) with the Sar1 GTPase at subdomains of the endoplasmic reticulum (ER) enables secretory cargoes to be concentrated efficiently within nascent transport intermediates, which subsequently deliver their contents to ER-Golgi intermediate compartments. Here, we define the spatiotemporal accumulation of native COPII subunits and secretory cargoes at ER subdomains under differing nutrient availability conditions using a combination of CRISPR/Cas9-mediated genome editing and live cell imaging. Our findings demonstrate that the rate of inner COPII coat recruitment serves as a determinant for the pace of cargo export, irrespective of COPII subunit expression levels. Moreover, increasing inner COPII coat recruitment kinetics is sufficient to rescue cargo trafficking deficits caused by acute nutrient limitation. Our findings are consistent with a model in which the rate of inner COPII coat addition acts as an important control point to regulate cargo export from the ER.
Identifiants
pubmed: 38066006
doi: 10.1038/s41467-023-44002-7
pii: 10.1038/s41467-023-44002-7
pmc: PMC10709328
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
8140Subventions
Organisme : U.S. Department of Health & Human Services | National Institutes of Health (NIH)
ID : GM134865
Organisme : U.S. Department of Health & Human Services | National Institutes of Health (NIH)
ID : GM008688
Organisme : U.S. Department of Health & Human Services | National Institutes of Health (NIH)
ID : S10RR025483
Informations de copyright
© 2023. The Author(s).
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