A Comparison of the Oral Microbiota in Healthy Dogs and Dogs with Oral Tumors.
DNA shotgun sequencing
biopsy
dogs
oral microbiota
oral swab
oral tumors
Journal
Animals : an open access journal from MDPI
ISSN: 2076-2615
Titre abrégé: Animals (Basel)
Pays: Switzerland
ID NLM: 101635614
Informations de publication
Date de publication:
21 Nov 2023
21 Nov 2023
Historique:
received:
09
08
2023
revised:
14
11
2023
accepted:
16
11
2023
medline:
9
12
2023
pubmed:
9
12
2023
entrez:
9
12
2023
Statut:
epublish
Résumé
The aim of this study was to further describe the oral microbiota of healthy dogs by DNA shotgun sequencing and compare those to dogs with oral tumors. Oral swabs (representative of all niches of the oral cavity) were collected from healthy dogs (n = 24) and from dogs with different oral tumors (n = 7). DNA was extracted from the swabs and shotgun metagenomic sequencing was performed. Only minor differences in microbiota composition were observed between the two groups. At the phylum level, the Bacteroidota, Proteobacteria, Actinobacteriota, Desulfobacterota and Firmicutes were most abundant in both groups. Observed Operational Taxonomic Units-OTUs (species richness) was significantly higher in the healthy patients, but there was no significant difference in the Shannon diversity index between the groups. No significant difference was found in beta diversity between the groups. The core oral microbiota consisted of 67 bacterial species that were identified in all 24 healthy dogs. Our study provides further insight into the composition of the oral microbiota of healthy dogs and in dogs with oral tumors.
Identifiants
pubmed: 38066947
pii: ani13233594
doi: 10.3390/ani13233594
pmc: PMC10705671
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Slovenian Research Agency
ID : P3-0428, P4-0053, J4-2546
Organisme : Purina PetCare Research Excellence Fund
ID : The microbiome research at the Gastrointestinal Laboratory at Texas A&M University is in part funded by Purina PetCare Research Excellence Fund
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