Expression of Tissue Factor and Platelet/Leukocyte Markers on Extracellular Vesicles Reflect Platelet-Leukocyte Interaction in Severe COVID-19.
COVID-19
extracellular vesicles
immunothrombosis
platelet–leukocyte aggregates
tissue factor
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
28 Nov 2023
28 Nov 2023
Historique:
received:
11
10
2023
revised:
21
11
2023
accepted:
24
11
2023
medline:
9
12
2023
pubmed:
9
12
2023
entrez:
9
12
2023
Statut:
epublish
Résumé
Severe COVID-19 is frequently associated with thromboembolic complications. Increased platelet activation and platelet-leukocyte aggregate formation can amplify thrombotic responses by inducing tissue factor (TF) expression on leukocytes. Here, we characterized TF-positive extracellular vesicles (EVs) and their cellular origin in 12 patients suffering from severe COVID-19 (time course, 134 samples overall) and 25 healthy controls. EVs exposing phosphatidylserine (PS) were characterized by flow cytometry. Their cellular origin was determined by staining with anti-CD41, anti-CD45, anti-CD235a, and anti-CD105 as platelet, leukocyte, red blood cell, and endothelial markers. We further investigated the association of EVs with TF, platelet factor 4 (PF4), C-reactive protein (CRP), and high mobility group box-1 protein (HMGB-1). COVID-19 patients showed higher levels of PS-exposing EVs compared to controls. The majority of these EVs originated from platelets. A higher amount of EVs in patient samples was associated with CRP, HMGB-1, PF4, and TF as compared to EVs from healthy donors. In COVID-19 samples, 16.5% of all CD41
Identifiants
pubmed: 38069209
pii: ijms242316886
doi: 10.3390/ijms242316886
pmc: PMC10707108
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Lower Austrian Society for Research Funding (GFF)
ID : K3-F-744/005-2019
Organisme : Technology Fund of Lower Austria
ID : WST3-F-5030664/032-2022
Références
Nat Rev Immunol. 2021 May;21(5):319-329
pubmed: 33824483
Atherosclerosis. 2020 Aug;307:109-120
pubmed: 32439204
Nat Commun. 2017 Jan 23;8:14188
pubmed: 28112148
Cells. 2021 Jan 07;10(1):
pubmed: 33430260
J Clin Invest. 2020 Nov 2;130(11):6151-6157
pubmed: 32759504
Ann Rheum Dis. 2014 Oct;73(10):1854-63
pubmed: 23873874
J Leukoc Biol. 2022 Jan;111(1):63-74
pubmed: 34730839
Res Pract Thromb Haemost. 2022 May 02;6(3):e12709
pubmed: 35509326
Res Pract Thromb Haemost. 2022 Mar 04;6(2):e12677
pubmed: 35284777
Arterioscler Thromb Vasc Biol. 2021 Feb;41(2):878-882
pubmed: 33267656
Clin Lipidol. 2009 Dec;4(6):767
pubmed: 20161667
Viruses. 2021 Nov 26;13(12):
pubmed: 34960645
J Extracell Vesicles. 2022 Apr;11(4):e12200
pubmed: 35362259
Blood. 2020 Sep 3;136(10):1169-1179
pubmed: 32597954
Cells. 2022 Aug 11;11(16):
pubmed: 36010572
Front Med (Lausanne). 2023 Jun 02;10:1186122
pubmed: 37332749
Nat Rev Immunol. 2013 Jan;13(1):34-45
pubmed: 23222502
Int J Mol Sci. 2023 Jan 18;24(3):
pubmed: 36768242
Front Hum Neurosci. 2017 Aug 03;11:390
pubmed: 28824397
Curr Opin Hematol. 2021 Nov 1;28(6):445-453
pubmed: 34232139
Vascul Pharmacol. 2022 Aug;145:106999
pubmed: 35597450
EBioMedicine. 2021 May;67:103369
pubmed: 33971404
Sci Rep. 2021 Mar 26;11(1):6996
pubmed: 33772103
Circ Res. 2020 Sep 17;:
pubmed: 32938299
Br J Haematol. 2021 Oct;195(1):90-94
pubmed: 34101171
Blood Adv. 2021 Feb 9;5(3):628-634
pubmed: 33560376
Circ Res. 2011 May 13;108(10):1284-97
pubmed: 21566224
Platelets. 2022 Feb 17;33(2):200-207
pubmed: 34260328
JACC Basic Transl Sci. 2021 Mar;6(3):202-218
pubmed: 33649738
Diagnostics (Basel). 2023 Mar 11;13(6):
pubmed: 36980378
Blood Coagul Fibrinolysis. 1998 Mar;9 Suppl 1:S9-14
pubmed: 9819023
Arterioscler Thromb Vasc Biol. 2014 Sep;34(9):1977-84
pubmed: 25012129
Front Immunol. 2022 Mar 02;13:838011
pubmed: 35309344
Arterioscler Thromb Vasc Biol. 2008 Aug;28(8):1491-8
pubmed: 18497306
Psychon Bull Rev. 2018 Feb;25(1):178-206
pubmed: 28176294
Blood. 2020 Sep 10;136(11):1330-1341
pubmed: 32678428
Int J Mol Sci. 2021 Apr 07;22(8):
pubmed: 33917210
Immunol Rev. 2022 Nov;312(1):61-75
pubmed: 35708588
Cardiovasc Res. 2012 Oct 1;96(1):64-72
pubmed: 22798388
Front Immunol. 2021 Dec 09;12:792448
pubmed: 34956225
Thromb Haemost. 2022 Jan;122(1):113-122
pubmed: 34077977
Blood. 2020 Sep 10;136(11):1317-1329
pubmed: 32573711
J Thromb Haemost. 2022 Feb;20(2):434-448
pubmed: 34710269
Thromb Haemost. 2020 Dec;120(12):1733-1735
pubmed: 33124027
Eur Heart J. 2015 Jun 7;36(22):1405-14
pubmed: 25660055
Int J Mol Sci. 2022 Feb 05;23(3):
pubmed: 35163743
Sci Rep. 2017 Jul 26;7(1):6522
pubmed: 28747771
Thromb Res. 2020 Jul;191:145-147
pubmed: 32291094
Semin Thromb Hemost. 2023 Jul;49(5):561-564
pubmed: 36781153
Blood Adv. 2022 Sep 13;6(17):5085-5099
pubmed: 35420680