Transient Receptor Potential Vanilloid 4-Dependent Microglial Function in Myelin Injury and Repair.

EAE TRPV4 cuprizone disease activity experimental autoimmune encephalitis inflammation microglia multiple sclerosis transient receptor potential vanilloid 4

Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
04 Dec 2023
Historique:
received: 18 09 2023
revised: 01 11 2023
accepted: 21 11 2023
medline: 9 12 2023
pubmed: 9 12 2023
entrez: 9 12 2023
Statut: epublish

Résumé

Microglia are found pathologically at all stages of multiple sclerosis (MS) lesion development and are hypothesized to contribute to both inflammatory injury and neuroprotection in the MS brain. Transient receptor potential vanilloid 4 (TRPV4) channels are widely expressed, play an important role as environmental sensors, and are involved in calcium homeostasis for a variety of cells. TRPV4 modulates myeloid cell phagocytosis in the periphery and microglial motility in the central nervous system. We hypothesized that TRPV4 deletion would alter microglia phagocytosis in vitro and lessen disease activity and demyelination in experimental autoimmune encephalitis (EAE) and cuprizone-induced demyelination. We found that genetic deletion of TRPV4 led to increased microglial phagocytosis in vitro but did not alter the degree of demyelination or remyelination in the cuprizone mouse model of MS. We also found no difference in disease in EAE following global or microglia-specific deletion of

Identifiants

pubmed: 38069420
pii: ijms242317097
doi: 10.3390/ijms242317097
pmc: PMC10706888
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NINDS NIH HHS
ID : R01NS106289
Pays : United States
Organisme : NINDS NIH HHS
ID : R25NS090978
Pays : United States

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Auteurs

Jameson P Holloman (JP)

Department of Neurology, Washington University School of Medicine, Saint Louis, MO 63110, USA.

Sophia H Dimas (SH)

Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

Angela S Archambault (AS)

Department of Neurology, Washington University School of Medicine, Saint Louis, MO 63110, USA.

Fabia Filipello (F)

Department of Neurology, Washington University School of Medicine, Saint Louis, MO 63110, USA.

Lixia Du (L)

Department of Anesthesiology, The Center for the Study of Itch and Sensory Disorders, Washington University School of Medicine, St. Louis, MO 63110, USA.

Jing Feng (J)

Department of Anesthesiology, The Center for the Study of Itch and Sensory Disorders, Washington University School of Medicine, St. Louis, MO 63110, USA.

Yonghui Zhao (Y)

Department of Anesthesiology, The Center for the Study of Itch and Sensory Disorders, Washington University School of Medicine, St. Louis, MO 63110, USA.

Bryan Bollman (B)

Department of Neurology, Washington University School of Medicine, Saint Louis, MO 63110, USA.

Laura Piccio (L)

Department of Neurology, Washington University School of Medicine, Saint Louis, MO 63110, USA.

Andrew J Steelman (AJ)

Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Department Neuroscience Program, Division of Nutritional Sciences, and Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

Hongzhen Hu (H)

Department of Anesthesiology, The Center for the Study of Itch and Sensory Disorders, Washington University School of Medicine, St. Louis, MO 63110, USA.

Gregory F Wu (GF)

Department of Neurology, Washington University School of Medicine, Saint Louis, MO 63110, USA.
Department of Pathology and Immunology, Washington University School of Medicine, Saint Louis, MO 63110, USA.

Classifications MeSH